p19 siRNA Binding Protein
icon_recomb 25 degree incubation temp

  • Catalog # M0310 was discontinued on January 02, 2020 ;
  • p19 siRNA Binding Protein (19kDa) binds siRNAs with nanomolar affinity
  • Used for the isolation of siRNAs
  • The dimeric protein preferentially binds 21-nucleotide siRNAs with a 2-nucleotide 3' extension and a 5' phosphate
  • Binds RNA in a size-dependent and sequence-independent manner
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  • Product Information
    The p19 siRNA Binding Protein (19 kDa) from the Carnation Italian Ringspot Virus (CIRV) plant binds siRNAs with nanomolar affinity(1). The dimeric protein binds 21 base siRNAs with a 2 base 3´ extension and a 5´phosphate. The protein binds RNA in a size dependent and sequence independent manner. If the siRNAs are 4 bases longer, the affinity for the protein is reduced about 100-fold (2). When p19 siRNA Binding Protein is expressed in plants it suppresses RNA interference (3).

    Figure 1: Figure 1

    Size specific binding of siRNA by p19 siRNA Binding Protein. Three phosphorylated dsRNAs of 17,21 and 25 bases (30 ng each band) were mixed with increasing amount of p19 siRNA Binding Protein (0-3 µg) in a 20 µl reaction, and incubated at room temperature for 1 hour. The reaction was analyzed on a 20% polyacrylamide gel stained with ethidium bromide. Marker M is the siRNA Marker (NEB #N2101 ).

    Highlights

    • For the isolation of siRNA
    • Isolated from a recombinant source

    Product Source

    p19 siRNA Binding Protein is cloned and expressed in E. coli as a fusion protein with an amino terminal MBP (maltose binding protein) and a carboxy terminal CBD (chitin binding domain).

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C) Concentration
    p19 siRNA Binding Buffer (200 μl) 2 X
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