DNase I (RNase-free)

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0303S     -20       DNase I (RNase-free) M0303SVIAL -20 1 x 0.5 ml 2,000 units/ml   DNase I Reaction Buffer B0303SVIAL -20 1 x 1.5 ml 10 X
M0303L     -20       DNase I (RNase-free) M0303LVIAL -20 2 x 1.25 ml 2,000 units/ml   DNase I Reaction Buffer B0303SVIAL -20 1 x 1.5 ml 10 X

Application Features

• Degradation of DNA template in transcription reactions
• Removal of contaminating genomic DNA from RNA samples
• DNase I footprinting
• Nick Translation

Materials Sold Separately

• DNase I Reaction Buffer

1. EDTA should be added to a final concentration of 5 mM to protect RNA from being degraded during enzyme inactivation (3).
2. We do not recommend using NEB's DNase I as a substitute for Monarch DNase I in the RNA isolation workflow when using the Monarch Total RNA Miniprep Kit (NEB #T2010).  Monarch DNase I is optimized for use in this workflow, and is available for purchase as a component of the Monarch Total RNA Enzyme Pack, (NEB #T2019).

1. Kunitz, M. (1950). J. Gen. Physiol . 33, 349-362.
2. Vanecko, S. and laskowski, M. (1961). J. Biol. Chem . 236, 3312-3316.
3. Huang, Z. et al. (1996). Biotechniques . 20, 1012-1020.

1. A Typical DNase I Reaction Protocol (M0303)

• Activity of DNA Modifying Enzymes in rCutSmart™ Buffer

• Activities of Exonucleases and Non-specific Endonucleases
• Common Applications for Exonucleases and Endonucleases
• Properties of Exonucleases and Non-specific Endonucleases

• Exo Selector

1. What is the specific activity of DNase I(RNase-free)?
2. Will DNase I work in NEB buffers 1-4?
3. What is the best way to remove DNase I from my reaction?
4. Will DNase I work in rCutSmart buffer?
5. Can I use the Monarch RNA Cleanup Kit to cleanup up my DNase I-treated RNA?
6. Can I use NEB DNase I (#M0303) with the Monarch Total RNA Miniprep Kit?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0303S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0303S_L_v1_0111403
• M0303S_L_v1_0111408
• M0303S_L_v1_0111501
• M0303S_L_v1_0141504
• M0303S_L_v1_0161506
• M0303S_L_v1_0111504
• M0303S_L_v1_0111503
• M0303S_L_v1_0161509
• M0303S_L_v1_0161601
• M0303S_L_v1_0161602
• M0303S_L_v1_0161605
• M0303S_L_v1_0161610
• M0303S_L_v1_0161701
• M0303S_L_v1_0181703
• M0303S_L_v1_0181710
• M0303S_L_v1_0191804
• M0303S_v1_10012894
• M0303S_v1_10018229
• M0303L_v1_10018233
• M0303S_v1_10021479
• M0303L_v1_10028566
• M0303S_v1_10029142
• M0303S_v1_10034586
• M0303L_v1_10036384
• M0303S_v1_10041611
• M0303L_v1_10043024
• M0303S_v1_10045754
• M0303L_v1_10049142
• M0303S_v1_10050912
• M0303L_v1_10053870
• M0303S_v1_10053872
• M0303L_v1_10057957
• M0303S_v1_10061040
• M0303L_v1_10062656
• M0303S_v1_10067106
• M0303L_v1_10067829
• M0303S_v1_10070933
• M0303L_v1_10075173
• M0303S_v1_10075174
• M0303L_v1_10079363
• M0303S_v1_10080105
• M0303S_v1_10082491
• M0303L_v1_10082490
• M0303L_v1_10088314
• M0303S_v1_10086168
• M0303L_v1_10090710
• M0303S_v1_10090711
• M0303L_v1_10098439
• M0303S_v1_10098442
• M0303S_v1_10106639
• M0303L_v1_10107431
• M0303S_v1_10107432
• M0303L_v1_10115312
• M0303S_v1_10115313
• M0303S_v1_10118363
• M0303L_v1_10118365
• M0303S_v1_10126209
• M0303L_v1_10130824
• M0303S_v1_10130825
• M0303L_v1_10133496
• M0303S_v1_10134726
• M0303S_v1_10140288
• M0303L_v1_10140286
• M0303L_v1_10145038
• M0303S_v1_10146838
• M0303L_v1_10150743
• M0303L_v1_10156732
• M0303S_v1_10150745
• M0303S_v1_10162927
• M0303S_v1_10166253
• M0303L_v1_10163652
• M0303L_v1_10171519
• M0303S_v1_10171297
• M0303S_v1_10171520
• M0303L_v1_10176122
• M0303S_v1_10180300
• M0303L_v1_10191393
• M0303S_v1_10191396

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• DNase I (RNase-free)
• DNase I Reaction Buffer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.