Topoisomerase I (E. coli) catalyzes the relaxation of negatively supercoiled DNA. Topoisomerase I has also been implicated in knotting and unknotting DNA (1) and in linking complementary rings of single-stranded DNA into double-stranded rings (2). The intact holoenzyme is a 97 kDa protein.
Product SourceAn E. coli strain containing the cloned topA gene
The following reagents are supplied with this product:
Store at (°C) Concentration CutSmart® Buffer -20 10X
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