T4 Gene 32 Protein
cloned at NEB recombinant NEB4 37 65 Heat

T4 Gene 32 Protein is a single-stranded DNA binding protein required for bacteriophage T4 replication and repair.

  • Used extensively to stabilize and mark regions of ssDNA for electron microscopic examination of intracellular DNA structure
  • Increase yield and processivity of reverse transcriptase during RT-PCR
  • Increase yield and specificity of PCR products from soil samples
Catalog # Concentration Size
M0300S 10 mg/ml 100 µg
M0300L 10 mg/ml 500 µg
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  • Product Information
    T4 Gene 32 Protein is a single-stranded DNA (ssDNA) binding protein required for bacteriophage T4 replication and repair (1-2). It cooperatively binds to and stablizes transiently formed regions of ssDNA and plays an important structural role during T4 phage replication (3). It also has been used extensively to stabilize and mark regions of ssDNA for electron microscopic examination of intracellular DNA structures (4-5). Recently, it has been shown to improve restriction enzyme digestion (6), improve the yield and efficiency of reverse transcription (RT) reactions during RT-PCR (7-9), enhance T4 DNA polymerase activity (10-11), as well as increase the yield of PCR products (12).

    Product Source

    An E. coli strain carrying a plasmid that overexpresses the gene 32 protein of T4 phage

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C) Concentration
    NEBuffer™ 4 -20 10 X
    Product Categories:
    ssDNA Binding Proteins Products
    Applications:
    Recombinase Polymerase Amplification and SIBA,
    RT-PCR & cDNA Synthesis
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