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McrBC
NEB2 37 65 Heat

McrBC is an endonuclease which cleaves DNA containing methylcytosine* on one or both strands. Sites on the DNA recognized by McrBC consist of two half-sites of the form (G/A)mC. These half-sites can be separated by up to 3 kb, but the optimal separation is 55-103 base pairs.

*5-methylcytosine or 5-hydroxymethylcytosine

  • McrBC requires GTP for DNA cleavage
  • Supplied with NEBuffer 2, Recombinant Albumin and 100x GTP

 

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Catalog # Concentration Size
M0272S 10,000 units/ml 500 units
M0272L 10,000 units/ml 2,500 units
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