- Also known as RNase T
- Single-stranded (ssDNA or RNA) specific exonuclease
- Catalyzes the removal of nucleotides from linear single-stranded DNA or RNA in the 3' to 5' direction
Exonuclease T can be used for:
- Removal of 3' overhangs of double-stranded DNA to generate blunt-ends*
- Removal of single-stranded primers in PCR reactions prior to DNA sequencing or SNP analysis
- Removal of single-stranded primers for nested PCR reactions
- Removal of single-stranded DNA, leaving behind double-stranded DNA in the sample
Exonuclease T (Exo T), also known as RNase T, is a single-stranded RNA (1,2) or DNA (3,4) specific nuclease that requires a free 3´ terminus and removes nucleotides in the 3´→ 5´ direction. Exonuclease T can be used to generate blunt ends from RNA (5) or DNA molecules that have 3´ extensions (2).
Product SourceExonuclease T is overexpressed and purified as a C-terminal fusion to maltose-binding protein (MBP). MBP is removed from Exonuclease T by Factor Xa cleavage and Exonuclease T is then purified away from Factor Xa and MBP. Exonuclease T cleaved from MBP has an additional amino acid on the N-terminus and a Phe instead of a Met (i.e., Glu-Phe-Exo T instead of Met-Exo T).
The following reagents are supplied with this product:
Store at (°C) Concentration NEBuffer™ 4 -20 10 X
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