Exonuclease T
icon_cloned icon_recomb icon_NEB4 25 degree incubation temp icon_HI_65

  • Also known as RNase T
  • Single-stranded (ssDNA or RNA) specific exonuclease
  • Catalyzes the removal of nucleotides from linear single-stranded DNA or RNA in the 3' to 5' direction

Exonuclease T can be used for:

  • Removal of 3' overhangs of double-stranded DNA to generate blunt-ends*
  • Removal of single-stranded primers in PCR reactions prior to DNA sequencing or SNP analysis
  • Removal of single-stranded primers for nested PCR reactions
  • Removal of single-stranded DNA, leaving behind double-stranded DNA in the sample


Request a copy of our Choosing the Right Exonuclease poster.

Learn more about how this product is being used in the Product Citation Tool
Catalog # Concentration Size
M0265S 5000 units/ml 250 units
M0265L 5000 units/ml 1250 units
To find out how to order this product from your current location, click the button below:
International Customer & Order Support
Please enter a quantity for at least one size
Loading Spinner