Exonuclease T
icon_cloned icon_recomb icon_NEB4 25 degree incubation temp icon_HI_65

  • Also known as RNase T
  • Single-stranded (ssDNA or RNA) specific exonuclease
  • Catalyzes the removal of nucleotides from linear single-stranded DNA or RNA in the 3' to 5' direction

Exonuclease T can be used for:

  • Removal of 3' overhangs of double-stranded DNA to generate blunt-ends*
  • Removal of single-stranded primers in PCR reactions prior to DNA sequencing or SNP analysis
  • Removal of single-stranded primers for nested PCR reactions
  • Removal of single-stranded DNA, leaving behind double-stranded DNA in the sample

*sequence-dependent

Request a copy of our Choosing the Right Exonuclease poster.

Learn more about how this product is being used in the Product Citation Tool
2019-20_Catalog_Promo_0519
Catalog # Concentration Size
M0265S 5000 units/ml 250 units
M0265L 5000 units/ml 1250 units
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