- DNA specific exonuclease
- Catalyzes the removal of nucleotides from linear single-stranded DNA in the 5' to 3' direction
- Preferred substrate is double-stranded DNA with 5' single stranded overhangs > 6 nucleotides long
RecJf can be used for:
- Degradation of single-stranded DNA from the 5'-end
- Removal of 5' protruding single-stranded termini at the ends of linear double-stranded DNA (blunt ends are not exclusively created)
RecJf is a single-stranded DNA specific exonuclease that catalyzes the removal of deoxy-nucleotide monophosphates from DNA in the 5' → 3' direction (1). RecJf is a recombinant fusion protein of RecJ and maltose binding protein (MBP). It has the same enzymatic properties as wild-type RecJ. Fusion to MBP enhances RecJf solubility (2).
When DNA containing a 22 base 5' extension is used as a substrate for RecJf, the resulting products are a mixture of DNA fragments that have blunt-ends, 5' extensions (1-5 nucleotides) and recessed 5' ends (1-8 nucleotides) (3). RecJf does not require a 5' phosphate (3).
Product SourceRecJf is overexpressed and purified as a C-terminal fusion to maltose-binding protein (MBP). MBP does not affect the catalytic activity of RecJf but does enhance RecJf solubility (2).
The following reagents are supplied with this product:
Store at (°C) Concentration NEBuffer™ 2 -20 10X
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