Vent® DNA Polymerase
Vent® DNA Polymerase offers moderate fidelity and robust performance
- Possesses 3´→ 5´ proofreading exonuclease activity
- 5X higher fidelity than Taq
- Difficult templates: ideal for GC-rich or looped sequence
- High thermostability: half-life of 6.7 hours at 95°C for maximum activity during PCR
Vent DNA Polymerase is a high-fidelity thermophilic DNA polymerase. The fidelity of Vent DNA Polymerase is 5-15-fold higher than that observed for Taq DNA Polymerase (1,2). This high fidelity derives in part from an integral 3'→5' proofreading exonuclease activity in Vent DNA Polymerase (1,3). Greater than 90% of the polymerase activity remains following a 1 hour incubation at 95°C.
Product SourceAn E. coli strain that carries the Vent DNA Polymerase gene from Thermococcus litoralis (4). The native organism is capable of growth at up to 98°C and was isolated from a submarine thermal vent (5).
The following reagents are supplied with this product:
Store at (°C) Concentration Magnesium Sulfate (MgSO4) Solution -20 100 mM ThermoPol® Reaction Buffer Pack -20 10 X
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