- DNA and RNA endonuclease
- Degrades double-stranded and single-stranded DNA and RNA
- Products are mononucleotides and oligonucleotides with terminal 3'-phosphates
Micrococcal Nuclease is ideal for:
- Preparation of double-stranded DNA fragments with 5'-OH and 3'-phosphate
- Studies of chromatin structure
- Degradation of nucleic acids in crude cell-free extracts
- Preparation of rabbit reticulocyte
Micrococcal nuclease is derived from Staphylococcus aureus and is a relatively non-specific endo-exonuclease. It is purified from a recombinant E. coli strain that digests double-stranded, single-stranded, circular and linear nucleic acids. The enzyme is active in the pH range of 7.0 - 10.0, with optimal activity at pH 9.2 for both RNA and DNA substrates. Cleavage preferences have been observed at sites rich in adenylate, deoxyadenylate or thymidylate (1). Both DNA and RNA are degraded to 3´ phosphomononucleotides and dinucleotides.
Micrococcal Nuclease works well with the SimpleChIP™ Enzymatic Chromatin IP Kit (Agarose Beads) #9002 from Cell Signaling Technology. Please follow the link to see product information and protocols on their website.
Product SourceAn E. coli strain containing a genetic fusion of the micrococcal nuclease gene (Gene ID: 3238436) and the gene coding for maltose binding protein, or MBP. The micrococcal nuclease is cleaved from the fusion protein and purified away from MBP.
The following reagents are supplied with this product:
Store at (°C) Concentration Micrococcal Nuclease Reaction Buffer 10X Purified BSA -20 10 mg/ml
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