9°N™ DNA Ligase
- Can be used to ligate nicks in DNA while incubating at high temperatures
- Extremely thermostable and can withstand PCR conditions
- Will not ligate short 4 base overlaps (typical of restriction enzyme digests), but efficiently ligates 12 base pair overlaps
- Isolated from a recombinant source
9° N™ DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between the 5´-phosphate and the 3´-hydroxyl of two adjacent DNA strands that are hybridized and accurately paired, with no gap, to a complementary DNA strand. 9° N DNA Ligase uses ATP as a cofactor and it is active at elevated temperatures (45° C – 70° C).
Product SourcePurified from an E. coli strain containing the cloned ligase gene from the extremely thermophilic marine archaea Thermococcus sp.(strain 9°N). The archaea was isolated from a submarine thermal vent, at a depth of 2,500 meters, 9° north of the equator at the East Pacific Rise (1).
The following reagents are supplied with this product:
Store at (°C) Concentration 9°N DNA Ligase Reaction Buffer 10 X
- Product Categories:
- DNA Ligases Products
- Cloning Ligation
- Protocols, Manuals & Usage
- Tools & Resources
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at [email protected] or 1-800-632-5227 x 8.