CpG Methyltransferase (M.SssI)
- Blocking restriction enzyme cleavage
- Studying of CpG methylation-dependent gene expression
- Probing sequence-specific contacts within the major groove of DNA
- Altering the physical properties of DNA
- Uniform (3H)-labeling of DNA
- Decreasing the number of sites cut by restriction endonucleases, yielding an apparent increase in specificity
The CpG Methyltransferase, M.SssI, methylates all cytosine residues (C5) within the double-stranded dinucleotide recognition sequence 5'...CG...3' (1).
Product SourceThe CpG Methyltransferase(M.SssI) is isolated from a strain of E. coli. which contains the Methyltransferase gene from Spiroplasma sp. strain MQ1 (2,3).
The following reagents are supplied with this product:
Store at (°C) Concentration NEBuffer™ 2 -20 10X S-adenosylmethionine (SAM) -20 32 mM
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