Human PRMT1 Methyltransferase

Materials Sold Separately

• S-adenosylmethionine (SAM)

1. Storage Note: S-adenosylmethionine (SAM) is stored at -20°C as a 32 mM solution dissolved in 0.005 M sulfuric acid and 10% ethanol (pH 7.5). Under these conditions, SAM is stable for up to 6 months. SAM is unstable at 37°C and should be replenished in reactions incubated longer than 4 hours. Methylation can be optimized by using fresh SAM. Avoid freeze/thaw of enzyme.

1. Tang, J. et al. (2000). J. Biol. Chem.. 275, 7723-7730.
2. Wang, H. et al. (2001). Science. 293, 853-857.
3. An, W. et al. (2004). Cell. 117, 735-748.

1. Can the histones be used as substrates for protein modification enzymes? Which ones?
2. Will any NEB methyltransferases (methylases) work on RNA?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Human PRMT1 Methyltransferase
• HMT Reaction Buffer
• S-adenosylmethionine (SAM)

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