Human PRMT1 Methyltransferase

Materials Sold Separately

• S-adenosylmethionine (SAM)

1. Storage Note: S-adenosylmethionine (SAM) is stored at -20°C as a 32 mM solution dissolved in 0.005 M sulfuric acid and 10% ethanol (pH 7.5). Under these conditions, SAM is stable for up to 6 months. SAM is unstable at 37°C and should be replenished in reactions incubated longer than 4 hours. Methylation can be optimized by using fresh SAM. Avoid freeze/thaw of enzyme.

1. Tang, J. et al. (2000). J. Biol. Chem.. 275, 7723-7730.
2. Wang, H. et al. (2001). Science. 293, 853-857.
3. An, W. et al. (2004). Cell. 117, 735-748.

1. Can the histones be used as substrates for protein modification enzymes? Which ones?
2. Will any NEB methyltransferases (methylases) work on RNA?

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The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Human PRMT1 Methyltransferase

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