DNA Polymerase I, Large (Klenow) Fragment
DNA Polymerase I, Large (Klenow) fragment was originally derived as a proteolytic product of E.coli DNA polymerase that retains polymerase and 3’ —> 5’ exonuclease activity
- Removal of 3’ overhangs or fill-in of 5’ overhangs to form blunt ends
- Lacks 5’ —> 3’ exonuclease activity
- Generates probes using random primers
- Second strand cDNA synthesis
DNA Blunting Tutorial
Traditional Cloning Workflow
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DNA Polymerase I, Large (Klenow) Fragment is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3'→ 5' exonuclease activity, but has lost 5'→ 3' exonuclease activity (1). Klenow retains the polymerization fidelity of the holoenzyme without degrading 5' termini.
Product SourceAn E. coli strain that contains the E. coli polA gene that has had its 5'→3' exonuclease domain removed.
The following reagents are supplied with this product:
Store at (°C) Concentration NEBuffer™ 2 -20 10 X
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