DNA Polymerase I (E. coli)
DNA Polymerase I (E. coli) is a DNA-dependent DNA polymerase with inherent 3′– 5´ and 5′– 3′ exonuclease activities
- Nick translation of DNA
- Second strand cDNA synthesis
DNA Polymerase I (E coli) is a DNA-dependent DNA polymerase with inherent 3´→ 5´ and 5´→ 3´ exonuclease activities (1). The 5´→ 3´ exonuclease activity removes nucleotides ahead of the growing DNA chain, allowing nick-translation.
Product SourceAn E. coli strain that carries an overexpressed copy of the polA gene.
The following reagents are supplied with this product:
Store at (°C) Concentration NEBuffer™ 2 -20 10X
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