Exonuclease III (E. coli)

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0206S     -20       Exonuclease III (E. coli) M0206SVIAL -20 1 x 0.05 ml 100,000 units/ml   NEBuffer™ 1 B7001SVIAL -20 1 x 1.25 ml 10 X
M0206L     -20       Exonuclease III (E. coli) M0206LVIAL -20 1 x 0.25 ml 100,000 units/ml   NEBuffer™ 1 B7001SVIAL -20 1 x 1.25 ml 10 X

Application Features

• Unidirectional nested deletions (3)
• Site-directed mutagenesis (6)
• Preparation of strand-specific probes (2)
• Preparation of single-stranded substrates for dideoxy sequencing (7)

Companion Products

• Monarch® Plasmid Miniprep Kit 
• Monarch® DNA Gel Extraction Kit
• Monarch® PCR & DNA Cleanup Kit (5 μg)

Materials Sold Separately

• NEBuffer 1

1. Phosphorothioate linkages are not cleaved by Exonuclease III. Unidirectional deletions can also be created by protecting one terminus by incorporation of α-phosphorothioate-containing nucleotide.

2. The activity of Exonuclease III in various buffers is listed below:

   • NEBuffer 1 - 100%
   • NEBuffer 2 - 75%
   • NEBuffer 3 -25%
   • NEBuffer 4 - 75%
   • CutSmart – 100%

1. Rogers, G.S. and Weiss, B. (1980). L. Grossman and K. Moldave(Ed.), Methods Enzymol.. 65, 201-211. New York: Academic Press.
2. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual (2nd. Ed.). 5.84-5.85.
3. Henikoff, S. (1984). Gene. 28, 351-359.
4. Richardson, C.C. et al. (1964). J. Biol. Chem. 239, 251-258.
5. Linxweiler, W. and Horz, W. (1982). Nucl. Acids Res. 10, 4845-4859.
6. Vandeyar, M.A. (1988). Gene. 65, 129-133.
7. Guo, L.H. and Wu, R. (1982). Nucl. Acids Res.. 10, 2065-2084.
8. Putney, S., Benkovic, S. and Schimmel, P. (1981). Proc. Natl. Acad. Sci. USA. 78, 7350-7354.

1. Protocol for Exonuclease III (M0206)

• Activities of Exonucleases and Non-specific Endonucleases
• Common Applications for Exonucleases and Endonucleases
• Properties of Exonucleases and Non-specific Endonucleases

• Exo Selector

• Choosing the Right Exonuclease (2019)

1. What is the activity of M0206 Exonuclease III in various NEB buffers?
2. How does T5 Exonuclease differ from Exonuclease III (NEB# M0206)?
3. How Does T7 Exonuclease differ from Exonuclease III (NEB# M0206)?
4. Will Exonuclease III work in rCutSmart buffer?
5. Can Exonuclease I be used with a double stranded exonuclease to clean up plasmid preparations?

• The effect of nucleic acid modifications on digestion by DNA exonucleases

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0206S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0206L_v1_10007562
• M0206S_v1_10007564
• M0206L_v1_10014353
• M0206_v0_10005610
• M0206S_v1_10022783
• M0206L_v1_10031189
• M0206S_v1_10025483
• M0206L_v1_10041628
• M0206S_v1_10047456
• M0206L_v1_10051787
• M0206S_v1_10055359
• M0206L_v1_10054866
• M0206L_v1_10063240
• M0206S_v1_10063242
• M0206L_v1_10075386
• M0206L_v1_10085452
• M0206S_v1_10089247
• M0206L_v1_10089249
• M0206L_v1_10097686
• M0206S_v1_10097685
• M0206S_v1_10103793
• M0206L_v1_10100276
• M0206S_v1_10115682
• M0206L_v1_10115684
• M0206L_v1_10133513
• M0206L_v1_10145031
• M0206L_v1_10155820
• M0206L_v1_10157411
• M0206S_v1_10156733
• M0206L_v1_10163813
• M0206L_v1_10167478
• M0206S_v1_10167475
• M0206L_v1_10182393

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Exonuclease III (E. coli)
• NEBuffer™ 1

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.