Exonuclease III (E. coli)
icon_recomb icon_NEB1 icon_incTemp37 icon_HI_70

  • Double-stranded DNA specific exonuclease
  • Initiates at the 3' termini of linear double-stranded DNA with 5' overhangs or blunt ends and 3' overhangs containing less than four bases
  • Initiates at nicked sites in double-stranded DNA
  • Catalyzes the removal of nucleotides from linear or nicked double-stranded DNA in the 3' to 5' direction

Exonuclease III is ideal for:

  • Site-directed mutagenesis
  • Preparation of single-stranded DNA for dideoxy sequencing
  • Preparation of nested deletions in double-stranded DNA

Request a copy of our Choosing the Right Exonuclease poster.

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Catalog # Concentration Size
M0206S 100,000 units/ml 5,000 units
M0206L 100,000 units/ml 25,000 units
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