T4 Polynucleotide Kinase
- 5' phosphorylation of DNA/RNA for subsequent ligation
- End labeling DNA or RNA for probes and DNA sequencing
- Removal of 3' phosphoryl groups
DNA Blunting Tutorial
Catalyzes the transfer and exchange of Pi from the γ position of ATP to the 5´ -hydroxyl terminus of polynucleotides (double-and single-stranded DNA and RNA) and nucleoside 3´-monophosphates. Polynucleotide Kinase also catalyzes the removal of 3´-phosphoryl groups from 3´-phosphoryl polynucleotides, deoxynucleoside 3´-monophosphates and deoxynucleoside 3´-diphosphates (1).
Product SourceA E. coli strain that carries the cloned T4 Polynucleotide Kinase gene. It is purified by a modification of the method of Richardson (1).
The following reagents are supplied with this product:
Store at (°C) Concentration T4 Polynucleotide Kinase Reaction Buffer -20 10X
- Phosphorylation (Kinase)
- Protocols, Manuals & Usage
- Tools & Resources
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at [email protected] or 1-800-632-5227 x 8.