- EnGen® Cas9 NLS, S. pyogenes: for genome engineering by direct introduction of active nuclease complexes
- EnGen® sgRNA Synthesis Kit: for rapid generation of microgram quantities of custom sgRNA
- EnGen® Mutation Detection Kit: for robust determination of targeting efficiency
- EnGen® Spy Cas9 Nickase: programmable site-specific DNA nicking for genome editing and in vitro applications
- EnGen Spy dCas9 (SNAP-tag): Programmable DNA binding activity compatible with broad variety of SNAP-tag conjugates
Protocols for Genome Editing
- EnGen® sgRNA Synthesis Kit, S. pyogenes Protocol (E3322)
- In vitro digestion of DNA with Cas9 Nuclease, S. pyogenes (M0386)
- T7 Endonuclease I-based Mutation Detection with the EnGen® Mutation Detection Kit (NEB #E3321)
- Transfection of Cas9 RNP (ribonucleoprotein) into adherent cells using the Lipofectamine® RNAiMAX
- Electroporation of Cas9 RNP (ribonucleoprotein) into adherent cells using the Neon® Electroporator
- Determining Genome Targeting Efficiency using T7 Endonuclease I
- Using recombinant Cas9 nuclease to assess locus modification in genome editing experiments (#M0386)
Genome Editing Brochure
Learn more about NEB's complete solution for your genome editing needs, starting with our new Cas9 Nuclease (S. pyogenes) for CRISPR studies.
- Kinetic Comparison of Cas9 Homologs Recognizing Diverse PAM Sequences (2018)
Other Tools & Resources
CRISPR systems evolved as a means of bacterial adaptive immunity. Understanding their behavior in vivo is essential to harnessing their power in vitro.
Get a high level overview of CRISPR/Cas9 and how it is used in genome editing in our Science in 60 segment.