
Methylation Dependent Restriction Enzymes for Epigenetics
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- What's the difference between DpnI, DpnII, MboI, and Sau3AI?
- How much enzyme should be used for cleaving genomic DNA?
- Does McrBC cut hemi-methylated DNA?
- Are there any published papers in which McrBC has been used?
- Will DpnI cleave hemimethylated DNA?
- Is extended digestion of McrBC recommended?
- Why does my McrBC cleaved DNA smear when run on an agarose gel?
- Does McrBC produce blunt or sticky ends?
- Protocol for generating 32 bp fragments from modified CpG sites in genomic DNA using LpnPI (R0663)
- Optimizing Restriction Endonuclease Reactions
- Double Digest Protocol with Standard Restriction Enzymes
- Protocol for Digestion Prior to droplet digital PCR (ddPCR)
- Protocol for Direct Digestion of gDNA during droplet digital PCR (ddPCR)
- Protocol for generating 32 bp fragments from modified CpG sites in genomic DNA using MspJI
- Protocol for generating 32 bp fragments from modified CpG sites in genomic DNA using FspEI
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Epigenetics - Expanding on Genomic Foundations
- Epigenetics Brochure
- Dam-Dcm and CpG Methylation
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Note: Yeast DNA does not contain methylated DNA and thus, no 32-mer is detected.
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