The EpiMark® line of products from New England Biolabs is designed to simplify DNA methylation and hydroxymethylation detection and analysis, as well as ChIP, histone and nucleosome analysis. This growing line of reagents and kits includes:
- EpiMark® Methylated DNA Enrichment Kit (NEB #E2600) to enrich double-stranded methyl-CpG from fragmented genomic DNA with as little as 5 ng of input DNA
- EpiMark® Bisulfite Conversion Kit (NEB #E3318) to detect methylated cytosine via the conversion of unmodified cytosines to uracil
- EpiMark® Hot Start Taq DNA Polymerase (NEB #M0490) to amplifiy bisulfite-treated samples
- EpiMark® 5-mC and 5-hmC Analysis Kit (NEB #E3317) to identify and quantify 5-hydroxymethylcytosine at specific loci
- EpiMark® Nucleosome Assembly Kit (NEB #E5350) to prepare recombinant nucleosomes
- NEBNext® Ultra II DNA Library Prep Kit for Illumina® (NEB #E7645) to prepare Chromatin immunoprecipitated DNA samples for next-generation sequencing
- NEBNext® Enzymatic Methyl-seq (EM-seq™) (NEB #E7120), an enzyme-based alternative to bisulfite sequencing for sensitive 5mC and 5hmC detection using next generation sequencing
EpiMark® and NEBNext® are registered trademarks of New England Biolabs, Inc.
EM-seq™ is a trademark of New England Biolabs, Inc.
Illumina® is a registered trademark of Illumina, Inc.
SOLiD™ is a trademark of Life Technologies, Inc.
- Capture and Elute Step for Control DNA (E2600)
- Capture Methylated CpG DNA (E2600)
- DNA Fragmentation (E2600)
- Downstream Analysis (NEB #E2600)
- Elute Captured Methylated CpG DNA (E2600)
- End-point PCR Using EpiMark Bisulfite Conversion Kit (E3318)
- Prebind MBD2a-Fc to Protein A Magnetic Beads (NEB #E2600)
- Reaction Protocol for EpiMark® 5-hmC and 5-mC Analysis Kit (E3317)
- Wash Off Unbound DNA (E2600)
- Dialysis Assembly Protocol (E5350)
- Dilution Assembly Protocol (E5350)
- Introduction (E5350)
- DNA Concentration Formulas for EpiMark™ Nucleosome Assembly Kit (E5350)
- EpiMark™ Nucleosome Assembly Kit (E5350)
- Gel Shift Assay for EpiMark™ Nucleosome Assembly Kit (E5350)
- Cycling Protocol Using EpiMark™ Bisulfite Conversion Kit (E3318)
- EpiMark® Hot Start Taq DNA Polymerase Guidelines for PCR (M0490)
- Reagent Preparation Using EpiMark® Bisulfite Conversion Kit (E3318)
- Optimizing Restriction Endonuclease Reactions
- Protocol for use with NEBNext ChIP-seq Library Prep Reagent Set for Illumina (E6200)
- Protocol for Glucosylation and digestion of Genomic DNA using AbaSI (#R0665)
- Protocol for generating 32 bp fragments from modified CpG sites in genomic DNA using MspJI, FspEI or LpnPI
- Protocol for Direct Digestion of gDNA during droplet digital PCR (ddPCR)
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
If all cells are created from the same genetic material, why are there so many different cell types? Listen to Sriharsa Pradhan, Senior Scientist, RNA Biology at NEB, as he describes how DNA is methylated and how this affects the path of reading the DNA code the same way an obstruction would derail a train off its tracks.