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Control DNA

Control DNAs are powerful tools in the investigation of genomic DNA methylation and epigenomic research. They can be utilized in many protocols where they can serve as comparisons to sample DNA. Both positive and negative methylation control DNA are available for bisulfite conversion methods, methyl-DNA immunoprecipitation (Me-DIP) or by methyl-CpG binding domain-based (MBDCap) proteins methylation-specific PCR (MSP) or real time PCR.

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Protocols for Control DNA
Applications
  • PCR
  • SNP analysis
  • Southern blotting
  • Genomic DNA library construction
  • Methylation-specific PCR (MSP)
  • Bisulfate sequencing
  • Methylation-sensitive single nucleotide primer extension (ms-SNUPE)
  • Combined bisulfite restriction analysis (COBRA)
  • Bisulfite treatment and PCR Single/Stranded Conformation Polymorphism Analysis (Bisulfite-PCR-SSCP/BiPS)
Legal Information

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.


Videos

  • What is Epigenetics?

    What Is Epigenetics?

    If all cells are created from the same genetic material, why are there so many different cell types? Listen to Sriharsa Pradhan, Senior Scientist, RNA Biology at NEB, as he describes how DNA is methylated and how this affects the path of reading the DNA code the same way an obstruction would derail a train off its tracks.

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