The NEBNext Ultra II RNA First Strand Synthesis Module has been optimized to convert a broad range of input amounts of RNA into cDNA using random priming.
The module is optimized for use with the NEBNext Ultra II Directional RNA Second Strand Synthesis Module (NEB #E7550) or the NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (NEB #E6111), and is part of the Ultra II RNA directional or non-directional workflows. These workflows are compatible with poly(A) mRNA isolation or ribosomal RNA depletion, and enable high yield preparation of high quality libraries from 5 ng – 1 µg total RNA.
The following reagents are supplied with this product:
Store at (°C) Concentration NEBNext First Strand Synthesis Reaction Buffer -20 Random Primers -20 NEBNext First Strand Synthesis Enzyme Mix -20 NEBNext Strand Specificity Reagent -20
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