NEBNext® ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies®)

Express protocol option now available, with reduced cleanup steps.
See below for additional NEBNext ARTIC kit options for Illumina platforms.

A bulk format (2,304 reactions) is also available. Please contact us for more information.

The NEBNext® ARTIC kits were developed in response to the critical need for reliable and accurate methods for SARS-CoV-2 sequencing. These kits, for long and short read sequencing, were based on the original work of the ARTIC Network(1). The ARTIC SARS-CoV-2 sequencing workflow is a multiplexed amplicon-based whole-viral-genome sequencing approach. 

NEBNext ARTIC kits include primers and reagents for RT-PCR from SARS-CoV-2 gRNA and downstream library preparation for Illumina® and Oxford Nanopore Technologies® sequencing. The V3 ARTIC primers have been balanced, using methodology developed at NEB based on empirical data from sequencing. In combination with optimized reagents for RT-PCR, the kits deliver improved uniformity of amplicon yields from gRNA across a wide copy number range. An Express protocol option with fewer cleanups is available.

The NEBNext ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies) is compatible with sequencing on the Oxford Nanopore Technologies (ONT) platform and is used alongside kits from ONT to generate libraries with inserts in the 400 bp range, that can be sequenced on any ONT instrument, in 2-24 hour run times. 

If Illumina-compatible libraries are preferred, the NEBNext ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina) (NEB #E7658) and the NEBNext ARTIC SARS-CoV-2 Library Prep Kit (Illumina) (NEB #E7650) generate libraries with ~150 bp and ~400 bp inserts, respectively. If only the reagents for cDNA synthesis and targeted cDNA amplification are required, the NEBNext ARTIC SARS-CoV-2 RT-PCR Module (NEB #E7626) is available.

  • Streamlined, high-efficiency protocol
  • Express protocol option available, with reduced cleanup steps
  • Effective with a wide range of viral genome inputs (10-10,000 copies)
  • Improved uniformity of SARS-CoV-2 genome coverage depth with a more balanced primer pool
  • Library inserts in the 400 bp range
  • Includes NEBNext Sample Purification Beads (SPRIselect®)
  • For a bulk format of the kit (2,304 reactions) please contact us

1.(Josh Quick 2020. nCoV-2019 sequencing protocol v2 (GunIt).

Catalog # Concentration Size
E7660S Not Applicable 24 reactions
E7660L Not Applicable 96 reactions
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