NEBNext ARTIC SARS-CoV-2 kits that also include library prep reagents are available for smaller (~150 bp) (NEB #E7658) or larger (~400 bp) (NEB #E7650) insert libraries for Illumina® sequencing, and for Oxford Nanopore Technologies® sequencing (NEB #E7660).
The NEBNext® ARTIC products were developed in response to the critical need for reliable and accurate methods for SARS-CoV-2 sequencing. These are based on the original work of the ARTIC Network(1) to develop a multiplexed amplicon-based whole-viral-genome sequencing approach.
NEBNext ARTIC SARS-CoV-2 RT-PCR Module contains the reagents required for cDNA synthesis and targeted cDNA amplification from SARS-CoV-2 genomic RNA. The balanced NEBNext ARTIC primers and optimized reagents deliver improved uniformity of amplicon yields from gRNA across a wide copy number range.
Full kits that also include library prep reagents optimized for the ARTIC workflow are available, for smaller (~150 bp) (NEB #E7658) or larger (~400 bp) (NEB #E7650) insert libraries for Illumina® sequencing, and for Oxford Nanopore Technologies® sequencing (NEB #E7660).
1. (Josh Quick 2020. nCoV-2019 sequencing protocol v2 (GunIt). protocols.io https://dx.doi.org/10.17504/protocols.io.bdp7i5rn).
The following reagents are supplied with this product:
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