This module is part of the Ultra™ II workflow, and is optimized for use with the NEBNext® Ultra II End Repair/dA-Tailing Module (NEB #E7546), for Illumina®-compatible library construction.
This module is also compatible with some Oxford Nanopore Technologies® workflows, including some involving COVID-19 research.
The NEBNext Ultra II Ligation Module has been optimized to ligate efficiently DNA adaptors compatible with Illumina sequencing to end-repaired, dA-tailed DNA fragments. The module is optimized for use with the NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546), and is part of the Ultra II DNA workflow which enables high yield preparation of high quality libraries from 500 pg to 1 µg of input DNA.
This module is compatible with Illumina workflows and with several Oxford Nanopore Technologies workflows.
For Illumina library construction, the NEBNext Ultra II Ligation Module is designed for use with the following:
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|NEBNext® Ligation Enhancer||-20|
|NEBNext® Ultra™ II Ligation Master Mix||-20|
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