The unique workflow of the NEBNext® Small RNA library prep kits addresses the challenge of minimization of adaptor-dimers while achieving production of high-yield, diverse multiplex libraries in a simple protocol.
The NEBNext Multiplex Small RNA Library Prep Kit for Illumina (Index Primers 1-48) contains the adaptors, primers, enzymes and buffers required to convert small RNAs into indexed libraries for next-generation sequencing on the Illumina platform. The novel workflow has been optimized to minimize adaptor-dimers, while producing high-yield, high-diversity libraries.
Each kit component must pass rigorous quality control standards, and each set of reagents is functionally validated together by construction and sequencing of indexed small RNA libraries on the Illumina sequencing platform.
For larger volume requirements, customized and bulk packaging is available by purchasing through the Custom Solutions department at NEB. Please contact [email protected] for further information.
NEBNext Multiplex Small RNA Library Prep Kit for Illumina (Index Primers 1-48)
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|Gel Loading Dye, Blue||25||6 X|
|ProtoScript® II Reverse Transcriptase||-20||200,000 units/ml|
|NEBNext 3´ Ligation Enzyme Mix||-20|
|NEBNext 3´ Ligation Reaction Buffer||-20||2 X|
|NEBNext 5´ Ligation Reaction Buffer||-20|
|NEBNext 5´ Ligation Enzyme Mix||-20|
|Murine RNase Inhibitor|
|LongAmp™ Taq 2X Master Mix||-20||2 X|
|NEBNext SR Primer for Illumina||-20|
|Quick-Load pBR322 DNA-MspI Digest||-20||50 µg/ml|
|DNA Gel Elution Buffer||-20||1 X|
|Linear Acrylamide||-20||10 mg/ml|
|NEBNext 5´ SR Adaptor for Illumina||-20|
|NEBNext 3´ SR Adaptor for Illumina||-20|
|NEBNext SR RT Primer for Illumina||-20|
|NEBNext First Strand Synthesis Reaction Buffer||-20||5 X|
|NEBNext Index 1-48 Primers for Illumina||-20|
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