NEBNext® Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina® library production. The unique hairpin loop structure of the NEBNext adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification. This kit includes a methylated version of the NEBNext adaptor, which is compatible with library preparation for bisulfite sequencing protocols.
Incorporating a novel hairpin loop structure, the NEBNext adaptor ligates with increased efficiency to end-repaired, dA-tailed DNA. The loop contains a U, which is removed by treatment with USER® Enzyme (a mix of UDG and Endo VIII), to open up the loop and make it available as a substrate for PCR. During PCR, barcodes can be incorporated by use of the NEBNext index primers, thereby enabling multiplexing.
NEBNext oligos can be used with NEBNext products, and with other standard Illumina-compatible library preparation protocols.
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