NEBNext® RNA First Strand Synthesis Module
However, we now recommend use of the more recent NEBNext Ultra II Directional RNA and RNA (non-directional) workflows, which have been further optimized to provide increased yields of high quality libraries from a broader range of input amounts, and a NEBNext Ultra II RNA First Strand Synthesis Module (NEB #E7771) is available.
The NEBNext RNA First Strand Synthesis Module is designed for synthesis of cDNA from RNA fragments using ProtoScript® II Reverse Transcriptase and random priming. Single strand cDNA can be used directly for second strand cDNA synthesis. For use in directional (strand-specific) protocols, Actinomycin D is required and must be purchased separately (see below).
The following reagents are supplied with this product:
Store at (°C) Concentration NEBNext First Strand Synthesis Reaction Buffer -20 Random Primers -20 ProtoScript® II Reverse Transcriptase -20 200000 units/ml Murine RNase Inhibitor -20
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