Don’t be biased! The NEBNext Ultra II DNA PCR-free Library Prep Kit for Illumina offers an amplification-free workflow for DNA-seq based on the streamlined and reliable Ultra II workflow. Starting with as little as 250 ng of DNA, get the high-quality, high-yield libraries that you need without PCR bias.
Note: If your input DNA has not already been sheared, we recommend the NEBNext Ultra II FS DNA PCR-free Library Prep Kit for Illumina, which fragments, end repairs, and dA-tails DNA, all in a single tube with no cleanups or transfers.
The NEBNext Ultra II DNA PCR-free Library Prep Kit contains the enzymes and buffers required to convert a broad range of sheared DNA input amounts into libraries for next-generation sequencing on the Illumina platform, all without an amplification step. The fast, user-friendly workflow requires minimal hands-on time, resulting in excellent library yields and GC coverage without requiring PCR.
For added convenience, this kit contains NEBNext Sample Purification Beads (SPRIselect beads from Beckman Coulter) for size selection and enzyme reaction clean-up steps.
A separate version of the NEBNext Ultra II DNA PCR-free Library Prep Kit is available without sample purification beads.
The following reagents are supplied with this product:
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