The unique workflow of the NEBNext® Small RNA library prep kits addresses the challenge of minimization of adaptor-dimers while achieving production of high-yield, diverse multiplex libraries in a simple protocol.
This version of the kit is intended for use with your barcode system, and please note that index primers for multiplexing are not included in this kit.
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|Gel Loading Dye, Blue||25||6 X|
|NEBNext 3´ Ligation Enzyme Mix||-20|
|NEBNext 3´ Ligation Reaction Buffer||-20||2 X|
|NEBNext 5´ Ligation Reaction Buffer||-20|
|NEBNext 5´ Ligation Enzyme Mix||-20|
|Murine RNase Inhibitor|
|LongAmp™ Taq 2X Master Mix||-20||2 X|
|NEBNext SR Primer for Illumina||-20|
|Quick-Load pBR322 DNA-MspI Digest||-20||50 µg/ml|
|DNA Gel Elution Buffer||-20||1 X|
|Linear Acrylamide||-20||10 mg/ml|
|NEBNext 5´ SR Adaptor for Illumina||-20|
|NEBNext Index 1 Primer for Illumina|
|NEBNext 3´ SR Adaptor for Illumina||-20|
|NEBNext SR RT Primer for Illumina||-20|
|NEBNext First Strand Synthesis Reaction Buffer||-20||5 X|
|ProtoScript II Reverse Transcriptase||-20||200,000 units/ml|
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