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NEBNext® Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs)

NEBNext Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs) is compatible with Illumina® sequencing and includes the NEBNext EM-seq™ Adaptor, and NEBNext PCR primers that enable incorporation of unique index pairs during library amplification.  The index primers are provided as pre-mixed unique pairs of i5 and i7 index primers, packaged in a single-use 96-well plate with a pierceable foil seal, or in individual vials.

Although optimized for the EM-seq workflow, the NEBNext EM-seq Adaptor is also compatible with bisulfite sequencing workflows.

These components are also included in the NEBNext Enzymatic Methyl-seq Kit (NEB #E7120).

  • Optimized for the EM-seq workflow
  • Compatible with bisulfite sequencing workflows
  • Addresses the “barcode hopping” issue seen with some Illumina sequencing instruments
  • Increases sample identification specificity with unique dual index primer pairs
Catalog # Concentration Size
E7140S Not Applicable 24 reactions
E7140L Not Applicable 96 reactions
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