NEBNext^® Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs)

Kit Components

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
E7140S     -20       EM-seq™ Index Primer 1 E7141AVIAL -20 1 x 0.005 ml 10 µM   EM-seq™ Index Primer 2 E7142AVIAL -20 1 x 0.005 ml 10 µM   EM-seq™ Index Primer 3 E7143AVIAL -20 1 x 0.005 ml 10 µM   EM-seq™ Index Primer 4 E7144AVIAL -20 1 x 0.005 ml 10 µM   EM-seq™ Index Primer 5 E7145AVIAL -20 1 x 0.005 ml 10 µM   EM-seq™ Index Primer 6 E7146AVIAL -20 1 x 0.005 ml 10 µM   EM-seq™ Index Primer 7 E7147AVIAL -20 1 x 0.005 ml 10 µM   EM-seq™ Index Primer 8 E7148AVIAL -20 1 x 0.005 ml 10 µM   EM-seq™ Index Primer 9 E7149AVIAL -20 1 x 0.005 ml 10 µM   EM-seq™ Index Primer 10 E7150AVIAL -20 1 x 0.005 ml 10 µM   EM-seq™ Index Primer 11 E7151AVIAL -20 1 x 0.005 ml 10 µM   EM-seq™ Index Primer 12 E7152AVIAL -20 1 x 0.005 ml 10 µM   EM-seq™ Index Primer 13 E7153AVIAL -20 1 x 0.005 ml 10 µM   EM-seq™ Index Primer 14 E7154AVIAL -20 1 x 0.005 ml 10 µM   EM-seq™ Index Primer 15 E7155AVIAL -20 1 x 0.005 ml 10 µM   EM-seq™ Index Primer 16 E7156AVIAL -20 1 x 0.005 ml 10 µM   EM-seq™ Index Primer 17 E7157AVIAL -20 1 x 0.005 ml 10 µM   EM-seq™ Index Primer 18 E7158AVIAL -20 1 x 0.005 ml 10 µM   EM-seq™ Index Primer 19 E7159AVIAL -20 1 x 0.005 ml 10 µM   EM-seq™ Index Primer 20 E7160AVIAL -20 1 x 0.005 ml 10 µM   EM-seq™ Index Primer 21 E7161AVIAL -20 1 x 0.005 ml 10 µM   EM-seq™ Index Primer 22 E7162AVIAL -20 1 x 0.005 ml 10 µM   EM-seq™ Index Primer 23 E7163AVIAL -20 1 x 0.005 ml 10 µM   EM-seq™ Index Primer 24 E7164AVIAL -20 1 x 0.005 ml 10 µM   NEBNext® EM-seq™ Adaptor E7165AVIAL -20 1 x 0.06 ml Not Applicable
E7140L     -20       NEBNext® 96 Unique Dual Index Primer Pairs Plate E7166AVIAL -20 1 x 5 μl/well 10 µM   NEBNext® EM-seq™ Adaptor E7165AAVIAL -20 1 x 0.24 ml Not Applicable

1. Setting up the PCR reactions (NEB #E7140)

• Forward Strand Workflow Sample Sheet for E7140L: NovaSeq 6000 with v1.0 reagent kits, MiniSeq with Rapid reagent kits, MiSeq, HiSeq 2000/2500 (paired-end flow cell), HiSeq 3000/4000 (single-read flow cell)
• Forward Strand Workflow Sample Sheet for E7140S: NovaSeq 6000 with v1.0 reagent kits, MiniSeq with Rapid reagent kits, MiSeq, HiSeq 2000/2500 (paired-end flow cell), HiSeq 3000/4000 (single-read flow cell)
• Reverse Complement Workflow Sample Sheet for E7140L: iSeq 100, MiniSeq with Standard reagent kits, NextSeq Systems, NovaSeq 6000 with v1.5 reagent kits, HiSeq 2000/2500 (single-read flow cell), HiSeq 3000/4000 (paired-end flow cell)
• Reverse Complement Workflow Sample Sheet for E7140S: iSeq 100, MiniSeq with Standard reagent kits, NextSeq Systems, NovaSeq 6000 with v1.5 reagent kits, HiSeq 2000/2500 (single-read flow cell), HiSeq 3000/4000 (paired-end flow cell)

• NEBNext® Multiplex Oligos Selection Chart

1. What is provided in the NEBNext Multiplex Oligos for Enzymatic Methyl-seq?
2. Can the NEBNext® Multiplex Oligos for Enzymatic Methyl-seq be used to address “index hopping” with Illumina patterned flow cells?
3. How many indices are available with the 24 reaction- or 96 reaction-size of the NEBNext® Multiplex Oligos for Enzymatic Methyl-seq?
4. Are the NEBNext® Multiplex Oligos for Enzymatic Methyl-seq the same as NEBNext® Multiplex Oligos for Illumina®?
5. Are the EM-seq Adaptor and Index Primers validated in the EM-seq workflow?
6. What is the concentration of the EM-seq Adaptor and Index Primers?
7. How many reactions worth of primer are in each well/tube?
8. Can I use the 96-well primer plate to do my PCR reactions?
9. Do I need to spike in custom sequencing primers when sequencing libraries made with NEBNext® Multiplex Oligos for Enzymatic Methyl-seq on Illumina sequencing instruments?
10. Can I perform single read runs and still get both index sequences? Can I run both single read and paired-end recipes with dual-indexed libraries?
11. Are Sample Sheets available for use with the NEBNext® Multiplex Oligos for EM-seq?
12. Can the EM-seq Adaptor be used for bisulfite sequencing?
13. Are dual indexed libraries compatible with single end sequencing?
14. What sequences need to be trimmed for NEBNext libraries that are sequenced on an Illumina instrument?

NEBNext Product Citations Search Tool

Use this tool to find citations related to NEBNext products. Search by product, keyword or instrument name – use the filters to select specific product areas.

• Enzymatic Methyl-seq: The Next Generation of Methylome Analysis

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• E7140G_v1
• E7140L_v1
• E7140S_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• E7140G_v0_10043558
• E7140L_v0_10043421
• E7140S_v0_10043544
• E7140G_v0_10045659
• E7140G_v1_10047000
• E7140G_v1_10047319
• E7140S_v1_10046861
• E7140G_v1_10039671
• E7140G_v1_10050488
• E7140S_v1_10050921
• E7140G_v1_10051938
• E7140S_v1_10053610
• E7140L_v1_10056050
• E7140G_v1_10056742
• E7140S_v1_10056749
• E7140L_v1_10057678
• E7140G_v1_10058151
• E7140G_v1_10058725
• E7140L_v1_10058649
• E7140S_v1_10058623
• E7140G_v1_10053416
• E7140S_v1_10061948
• E7140L_v1_10064734
• E7140S_v1_10065075
• E7140L_v1_10053436
• E7140L_v1_10066467
• E7140S_v1_10053424
• E7140G_v1_10070675
• E7140G_v1_10075734
• E7140G_v1_10087241
• E7140S_v1_10088730
• E7140L_v1_10092891
• E7140L_v1_10068764
• E7140S_v1_10093978
• E7140L_v1_10097950
• E7140S_v1_10097724
• E7140L_v1_10101019
• E7140S_v1_10068752
• E7140L_v1_10083308
• E7140G_v1_10104931
• E7140S_v1_10107185
• E7140S_v1_10108175
• E7140L_v1_10102629
• E7140L_v1_10111765
• E7140G_v1_10111750
• E7140S_v1_10102602
• E7140L_v1_10108750
• E7140S_v1_10118340
• E7140G_v1_10115817
• E7140L_v1_10120467
• E7140L_v1_10126613
• E7140G_v1_10113662
• E7140S_v1_10129944
• E7140L_v1_10132086
• E7140L_v1_10133597
• E7140S_v1_10135339
• E7140L_v1_10138518
• E7140L_v1_10127122
• E7140S_v1_10113670
• E7140G_v1_10143415
• E7140S_v1_10126995
• E7140G_v1_10149118
• E7140L_v1_10150431
• E7140L_v1_10153964
• E7140S_v1_10155612
• E7140G_v1_10157837
• E7140L_v1_10160406
• E7140S_v1_10160864
• E7140L_v1_10142157
• E7140S_v1_10142017
• E7140L_v1_10167933
• E7140S_v1_10168738
• E7140G_v1_10126987
• E7140S_v1_10158619
• E7140L_v1_10175487
• E7140S_v1_10181546
• E7140G_v1_10181694
• E7140L_v1_10158754
• E7140G_v1_10142009
• E7140S_v1_10183056
• E7140G_v1_10158611
• E7140L_v1_10185331
• E7140L_v1_10190016
• E7140S_v1_10190346

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• EM-seq™ Index Primer 1 (S)
• EM-seq™ Index Primer 2 (S)
• EM-seq™ Index Primer 3 (S)
• EM-seq™ Index Primer 4 (S)
• EM-seq™ Index Primer 5 (S)
• EM-seq™ Index Primer 6 (S)
• EM-seq™ Index Primer 7 (S)
• EM-seq™ Index Primer 8 (S)
• EM-seq™ Index Primer 9 (S)
• EM-seq™ Index Primer 10 (S)
• EM-seq™ Index Primer 11 (S)
• EM-seq™ Index Primer 12 (S)
• EM-seq™ Index Primer 13 (S)
• EM-seq™ Index Primer 14 (S)
• EM-seq™ Index Primer 15 (S)
• EM-seq™ Index Primer 16 (S)
• EM-seq™ Index Primer 17 (S)
• EM-seq™ Index Primer 18 (S)
• EM-seq™ Index Primer 19 (S)
• EM-seq™ Index Primer 20 (S)
• EM-seq™ Index Primer 21 (S)
• EM-seq™ Index Primer 22 (S)
• EM-seq™ Index Primer 23 (S)
• EM-seq™ Index Primer 24 (S)
• NEBNext^® EM-seq™ Adaptor
• NEBNext^® 96 Unique Dual Index Primer Pairs Plate (L)

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.