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NEBNext® Enzymatic Methyl-seq Kit

Note: This product now includes an additional component, and the protocols have been updated.

NEBNext Enzymatic Methyl-seq (EM-seq™) is a new method for identification of 5-mC and 5-hmC.

While bisulfite sequencing has been the gold standard for methylome analysis, this conversion treatment damages DNA, resulting in fragmentation, loss and bias. 

In contrast, the highly effective enzyme-based conversion in the NEBNext Enzymatic Methyl-seq Kit minimizes damage to DNA and, with the supplied NEBNext Ultra™ II library preparation workflow reagents, produces high quality libraries that enable superior detection of 5-mC and 5-hmC from fewer sequencing reads.

  • Superior sensitivity of detection of 5-mC and 5-hmC 
  • Greater mapping efficiency
  • More uniform GC coverage 
  • Detection of more CpGs with fewer sequence reads
  • Uniform dinucleotide distribution
  • High-efficiency library preparation, with larger library insert sizes
  • Conversion module also available separately

View or download extensive performance data in our Technical Note.

Catalog # Concentration Size
E7120S Not Applicable 24 reactions
E7120L Not Applicable 96 reactions
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