Even more from less, for DNA
Are you challenged with trying to obtain higher library yields using ever-decreasing input amounts? Each component in the NEBNext Ultra II DNA Library Prep Kit from NEB has been reformulated, resulting in a several-fold increase in library yield with as little as 500 picograms of input DNA. These advances deliver unprecedented performance, while enabling lower inputs and fewer PCR cycles.
Note: If your input DNA has not already been sheared, we recommend the NEBNext Ultra II FS DNA Library Prep Kit for Illumina, which fragments, end repairs, and dA-tails DNA, all in a single tube with no cleanups or transfers. This kit is available with and without sample purification beads.
To learn more about how NEBNext Ultra II addresses lower input amounts and challenging sample types, download our technical note.
For use with NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA Set 1) (NEB #E7395), refer to the Protocols tab for UMI Adaptors-specific guidance.
The following reagents are supplied with this product:
|NEB #||Component Name||Component #||Stored at (°C)||Amount||Concentration|
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