PURExpress® Δ RF123 kit is a variation of the PURExpress In vitro Protein Synthesis Kit where the release factors 1, 2 and 3 are omitted from the translation mix.
A rapid method for gene expression analysis, PURExpress® is a novel cell-free transcription/translation system reconstituted from the purified components necessary for E. coli translation. With minimal nuclease and protease activity, the PURExpress system preserves the integrity of DNA and RNA templates/complexes and results in proteins that are free of modification and degradation. Transcription and translation are carried out in a one-step reaction, and require the mixing of only two tubes. With results available in a few hours, PURExpress saves valuable laboratory time and is ideal for high throughput technologies.
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|PURExpress Solution A|
|Control (DHFR) template (10 μl)|
|Solution B (75 μl)||3.3 X|
|RF1 (10 μl)|
|RF2 (10 μl)|
|RF3 (10 μl)|
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