PURExpress® Δ (aa, tRNA) Kit
PURExpress® Δ (aa, tRNA) Kit is a variation of the PURExpress In vitro Protein Synthesis Kit where the amino acids and tRNA are omitted from the translation mix.
- Control amino acids and tRNA are provided separately
- Ideal for studies requiring control of the concentration or content of amino acids and/or tRNAs in a translation reaction
- Applications include non-natural amino acid incorporation, amino acid tRNA synthetase studies and labeling with amino acids that contain stable isotopes
A rapid method for gene expression analysis, PURExpress® is a novel cell-free transcription/translation system reconstituted from the purified components necessary for E. coli translation. With minimal nuclease and protease activity, the PURExpress system preserves the integrity of DNA and RNA templates/complexes and results in proteins that are free of modification and degradation. Transcription and translation are carried out in a one-step reaction, and require the mixing of only two tubes. With results available in a few hours, PURExpress saves valuable laboratory time and is ideal for high throughput technologies.
The following reagents are supplied with this product:
Store at (°C) Concentration PURExpress Solution A (-aa, tRNA) Control (DHFR) template (10 μl) E. coli tRNA (25 μl) Amino Acid Mix (25 μl) Solution B (75 μl) 3.3 X
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