PURExpress® In Vitro Protein Synthesis Kit

PURExpress® is a reconstituted protein synthesis system based on the PUREsystem™ (Shimizu et al., 2001) where all necessary components needed for in vitro transcription and translation are purified from E. coli.

  • Defined system with all his-tagged proteins for coupled transcription/translation; Ribosome is not his-tagged
  • Minimal nuclease and protease activity
  • Protein of interest can be synthesized and visualized in a few hours
  • Simple protocol and easy setup with Solutions A and B
  • Templates can be either plasmid DNA, linear DNA or mRNA
  • Synthesized protein can be co-translationally radiolabeled or fluorescently labeled
  • Protein can be reverse-purified or subject to direct functional analysis
  • Applications include high throughput screening/directed evolution, synthetic biology, toxic or difficult to express protein synthesis, studies on protein folding, activity and protein-protein interactions
  • Due to reconstituted nature several kits are offered where translation factors or macromolecules have been omitted to facilitate specific studies (see companion products)
Learn more about how this product is being used in the Product Citation Tool
Catalog # Concentration Size
E6800S Not Applicable 10 reactions
E6800L Not Applicable 100 reactions
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