PURExpress® In Vitro Protein Synthesis Kit

PURExpress® is a reconstituted protein synthesis system based on the PUREsystem™ (Shimizu et al., 2001) where all necessary components needed for in vitro transcription and translation are purified from E. coli.

•    Defined system with all his-tagged proteins for coupled transcription/translation; Ribosome is not his-tagged
•    T7 RNA Polymerase drives in vitro transcription
•    Minimal nuclease and protease activity for stability of synthesized protein and encoding target
•    Templates can be either plasmid DNA, linear DNA or mRNA
•    Protein of interest can be synthesized and visualized in a few hours
•    Synthesizes various target peptides and proteins
•    Synthesized protein can be co-translationally radiolabeled or fluorescently labeled
•    Protein can be reverse-purified or subject to direct functional analysis
•    Applications include high throughput screening/directed evolution, synthetic biology, toxic or difficult to express protein synthesis, studies on protein folding, activity and protein-protein interactions
•    Due to reconstituted nature, several kits are offered where translation factors or macromolecules have been omitted to facilitate specific studies (see companion products)
•    Compatible with the PURExpress Disulfide Bond Enhancer (NEB #E6820)
Learn more about how this product is being used in the Product Citation Tool
2019-20_Catalog_Promo_0519
Catalog # Concentration Size
E6800S Not Applicable 10 reactions
E6800L Not Applicable 100 reactions
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