NEBNext Direct® BRCA1/BRCA2 Panel
Target with precision.
NEBNext Direct employs a unique hybridization-based enrichment workflow that hybridizes baits directly to genomic DNA, without the need for upfront library preparation. The BRCA1/BRCA2 panel demonstrates extremely high specificity and unmatched coverage uniformity across a wide range of DNA inputs, allowing highly sensitive calling of germline and somatic variants while maximizing sequencer efficiency.
- Generate full (100%) coverage of all protein coding regions in BRCA1 and BRCA2 genes
- Obtain highly uniform sequencing across exon targets- 100% of base pairs in panel have coverage greater than 20% of the mean target coverage
- Maximize efficiency with a 1-day workflow that combines highly specific enrichment with library preparation
- Produce high depths of target coverage across a wide range of DNA input amounts for germline and somatic variant calling
Challenges and Opportunities for Next Generation Sequencing Target Enrichment
NEBNext Direct® Target Enrichment
NEBNext Direct® Workflow
NEB TV Ep. 15 – Applications of Restriction Enzymes
The NEBNext Direct BRCA1/BRCA2 Panel enriches the complete exon content of BRCA1 and BRCA2 genes for next-generation sequencing analysis. NEBNext Direct employs a unique hybridization-based enrichment workflow that hybridizes baits directly to genomic DNA, without the need for upfront library preparation. The BRCA1/BRCA2 panel demonstrates extremely high specificity and unmatched coverage uniformity across a wide range of DNA inputs, allowing highly sensitive calling of germline and somatic variants while maximizing sequencer efficiency.
NEBNext Direct® BRCA1/BRCA2 Resources:
- GRCh37hg19 Bed Files for the NEBNext Direct® BRCA1/BRCA2 Panel
- GRCh38hg38 Bed Files for the NEBNext Direct® BRCA1/BRCA2 Panel
- Protocol for NEBNext Direct BRCA1/BRCA2 Panel (E6627)
The following reagents are supplied with this product:
Store at (°C) Concentration NEBNext Sample Purification Beads NEBNext Direct BRCA1/BRCA2 Baits -20 NEBNext Direct® Bead Prep Buffer 4 NEBNext Direct® Hybridization Buffer 4 NEBNext Direct® dA-Tailing Buffer 4 NEBNext Direct® Adaptor Ligation Buffer 4 NEBNext Direct® Cleaving Buffer 4 NEBNext Direct® Hybridization Wash (HW) 4 NEBNext Direct® Bead Wash 1 25 NEBNext Direct® Bead Wash 2 4 NEBNext Direct® 3´ Adaptor -20 NEBNext Direct® 5´ UMI Adaptor -20 NEBNext Direct® dA-Tailing Enzyme -20 NEBNext Direct® Ligase -20 NEBNext Direct® 5´ Blunting Enzyme Mix -20 NEBNext Direct® Cleaving Enzyme Mix -20 NEBNext Direct® Q5 Master Mix -20 NEBNext Direct® FFPE Phosphorylation Enzyme -20 NEBNext Direct® Streptavidin Beads 4 NEBNext Direct® Hybridization Additive -20 NEBNext Direct® 5´ Blunting Buffer 4 NEBNext Direct® 3´ Blunting Enzyme Mix -20
- Product Categories:
- Targeted Enrichment
- FAQs & Tech Tips
- Protocols & Manuals
- Other Tools & Resources
- Quality & Safety
- Legal Information
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at email@example.com or 1-800-632-5227 x 8.