NEBNext® is now available for single cell and ultra-low RNA inputs!
This unique cDNA synthesis and amplification workflow meets the demand for a highly sensitive, yet robust method that consistently ultimately enables generation of high quality sequencing data from single cell or ultra-low input RNA.
Please note that this module contains only reagents for cDNA synthesis and amplification. The NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina® (NEB #E6420) includes reagents for DNA library construction.
The NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification Module uses a template switching method to generate full length cDNAs directly from single cells or 2 pg – 200 ng RNA. This unique workflow enables generation of high quality sequencing data from a broad range of inputs, and superior transcript detection, while providing reliably consistent performance.
Download extensive performance data in our technical note.
View extensive performance data in our technical note.
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further information.
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|NEBNext® Single Cell RT Primer Mix||-20|
|NEBNext® Single Cell RT Buffer||-20|
|NEBNext® Template Switching Oligo||-20|
|NEBNext® Single Cell RT Enzyme Mix||-20|
|NEBNext® Single Cell cDNA PCR Master Mix||-20|
|NEBNext® Single Cell cDNA PCR Primer||-20|
|NEBNext® Cell Lysis Buffer||-20|
|Murine RNase Inhibitor||-20|
|NEBNext® Bead Reconstitution Buffer||-20|
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