The NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent® includes enzymatic DNA fragmentation in a streamlined, high-performance library prep workflow.
The NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent® includes a master mix of reagents for enzymatic DNA fragmentation combined with end repair. This enables efficient and tunable DNA shearing and blunting with minimized sample loss before performing adaptor ligation and high-fidelity library amplification. The kit is compatible with input amounts from 10 ng - 1µg, with a workflow time of under 2 hours and ~12 min. hands-on time.Adaptors and primers for singleplex libraries are supplied in the kits. For multiplexed libraries, the Ion Xpress™ Barcode Adapters from Thermo Fisher Scientific can be used.
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|Bst 2.0 WarmStart® DNA Polymerase||-20|
|NEBNext DNA Fragmentation Master Mix||-20|
|NEBNext DNA Fragmentation Reaction Buffer||-20||10 X|
|T4 DNA Ligase||-20|
|NEBNext DNA Library Adaptors for Ion Torrent||-20|
|NEBNext DNA Library Primers for Ion Torrent™||-20||1 X|
|NEBNext Stop Buffer||-20||10 X|
|Magnesium Chloride (MgCl2) (200 mM)||-20|
|T4 DNA Ligase Buffer for Ion Torrent||-20||10 X|
|TE Buffer||-20||0.1 X|
|NEBNext Q5 Hot Start HiFi PCR Master Mix||-20||2 X|
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