NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina®
Please note that for ChIP DNA library construction we now recommend the NEBNext® Ultra™ II DNA Library Prep Kit (NEB #E7645).
The NEBNext ChIP-Seq Library Prep Kit for Illumina® utilizes the original standard library prep workflow, which is longer than the NEBNext Ultra II DNA Library Prep Kit workflow, has more components, requires more clean-up steps and higher input amounts.
The NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina contains reagents for preparation of libraries for next-generation sequencing on the Illumina platform from 10 ng of ChIP DNA, in a standard workflow. Please note that adaptors and primers are not included in the kit and are available separately.
The following reagents are supplied with this product:
Store at (°C) Concentration NEBNext High-Fidelity 2X PCR Master Mix (SAMPLE) 2 X NEBNext dA- Tailing Reaction Buffer 10 X Klenow Fragment (3´→5´ exo-) Quick T4 DNA Ligase -20 NEBNext End Repair Enzyme Mix NEBNext End Repair Reaction Buffer 10 X NEBNext Quick Ligation Reaction Buffer -20 5 X NEBNext® Q5® Hot Start HiFi PCR Master Mix -20
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