In place of E6240 (discontinued on January 02 2020), the following products can be used together, in the same protocol:
NEBNext® End Repair Module (NEB #E6050)
NEBNext® dA-Tailing Module (NEB #E6053)
NEBNext® Quick Ligation Module (NEB #E6056)
NEBNext® Q5® HotStart HiFi PCR Master Mix (NEB #M0543)
Please note that for ChIP DNA library construction we now recommend the NEBNext® Ultra™ II DNA Library Prep Kit (NEB #E7645, #E7103).
The NEBNext ChIP-seq Library Prep Kit for Illumina® utilizes the original standard library prep workflow, which is longer than the NEBNext Ultra II DNA Library Prep Kit workflow, has more components, requires more clean-up steps and higher input amounts.
Storage Temperature: -20°C
The NEBNext ChIP-seq Library Prep Master Mix Set for Illumina contains reagents for preparation of libraries for next-generation sequencing on the Illumina platform from 10 ng of ChIP DNA, in a standard workflow. Please note that adaptors and primers are not included in the kit and are available separately.
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