Please note that for ChIP DNA library construction we now recommend the NEBNext® Ultra™ II DNA Library Prep Kit (NEB #E7645).
The NEBNext ChIP-seq Library Prep Kit for Illumina® utilizes the original standard library prep workflow, which is longer than the NEBNext Ultra II DNA Library Prep Kit workflow, has more components, requires more clean-up steps and higher input amounts.
The NEBNext ChIP-seq Library Prep Master Mix Set for Illumina contains reagents for preparation of libraries for next-generation sequencing on the Illumina platform from 10 ng of ChIP DNA, in a standard workflow. Please note that adaptors and primers are not included in the kit and are available separately.
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|NEBNext High-Fidelity 2X PCR Master Mix (SAMPLE)||2 X|
|NEBNext dA- Tailing Reaction Buffer||10 X|
|Klenow Fragment (3´→5´ exo-)|
|Quick T4 DNA Ligase||-20|
|NEBNext End Repair Enzyme Mix|
|NEBNext End Repair Reaction Buffer||10 X|
|NEBNext Quick Ligation Reaction Buffer||-20||5 X|
|NEBNext® Q5® Hot Start HiFi PCR Master Mix||-20|
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