NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina®


Please note that for ChIP DNA library construction we now recommend the NEBNext® Ultra™ II DNA Library Prep Kit (NEB #E7645).

The NEBNext ChIP-Seq Library Prep Kit for Illumina® utilizes the original standard library prep workflow, which is longer than the NEBNext Ultra II DNA Library Prep Kit workflow, has more components, requires more clean-up steps and higher input amounts.

Catalog # Concentration Size
E6240S Not Applicable 12 reactions
E6240L Not Applicable 60 reactions
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  • Product Information

    The NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina contains reagents for preparation of libraries for next-generation sequencing on the Illumina platform from 10 ng of ChIP DNA, in a standard workflow. Please note that adaptors and primers are not included in the kit and are available separately.

    ChIP DNA Library Preparation Workflow for Illumina
    ChIP DNA Library Preparation Workflow for Illumina

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C) Concentration
    NEBNext High-Fidelity 2X PCR Master Mix (SAMPLE) 2X
    NEBNext dA- Tailing Reaction Buffer -20 10X
    Klenow Fragment (3´→5´ exo-) -20
    Quick T4 DNA Ligase -20
    NEBNext End Repair Enzyme Mix -20
    NEBNext End Repair Reaction Buffer -20 10X
    NEBNext Quick Ligation Reaction Buffer -20 5X
    NEBNext® Q5® Hot Start HiFi PCR Master Mix -20
    Product Categories:
    ChIP-Seq Library Prep Products
    DNA Sequencing,
    DNA for Illumina
    Illumina Library Preparation
    • Properties & Usage
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