Refer to your specific sample preparation protocol to determine conditions for use.
The NEBNext DNA Library Prep Reagent Set for 454 contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing (1), and for preparation of single stranded DNA for use in high density hybridization arrays (2) or for genomic subtraction hybridization methods (3). Each of these components must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further information.
Functional ValidationEach set of reagents is functionally validated together through construction and sequencing of a transcriptome library and sequenced on a RNA library on the 454 platform,DNA library on the 454 platform
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