EpiMark® Nucleosome Assembly Kit
The Epimark Nucleosome Assembly Kit is used to make unmodified recombinant human nucleosomes with user-supplied DNA or provided control DNA.
- Highly pure, recombinant proteins
- Pre-formed histone dimer and tetramer complexes simplify octamer formation
- Components stable for one year
- Dilution protocol requires only a few hours for assembly
- Applications include ChIP, HAT and enzymatic modification assays
The EpiMark® Nucleosome Assembly Kit is used to make unmodified recombinant human nucleosomes with user-supplied DNA or the provided control DNA. Purified recombinant human Histone H2A/H2B Dimer and Histone H3.1/H4 Tetramer are mixed with DNA at 2 M NaCl. To generate nucleosomes, the salt concentration is lowered by dilution or dialysis allowing each histone tetramer to associate with two histone dimers and form the histone octamer on the DNA (1,2). A method for assaying nucleosome formation by gel shift assay is included. These nucleosomes may serve as better substrates for enzymes that are inactive on the DNA or one of the core histones alone (3,4). Each reaction creates nucleosomes from ~1 µg of 208 bp DNA and may be scaled depending on the experiment.
The following reagents are supplied with this product:
Store at (°C) Concentration Histone H2A/H2B Dimer Human, Recombinant -20 20 µM Histone H3.1/H4 Tetramer Human, Recombinant -20 10 µM Nucleosome Control DNA -20 10 µM
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