The EpiMark® 5-hmC and 5-mC Analysis Kit can be used to analyze and quantitate 5-methylcytosine and 5-hydroxymethylcytosine within a specific locus. Complete conversion of 5-hmC to glucosylated 5-hmC in DNA by T4 β-glucosyltransferase (T4-BGT).
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|NEBuffer™ 4||-20||10 X|
|T4 Phage β-glucosyltransferase (T4-BGT)||-20||10,000 units/ml|
|Unmodified Control DNA||-20||0.1 ng/µl|
|5-mC Control DNA||-20||0.1 ng/µl|
|5-hmC Control DNA||-20||0.1 ng/µl|
|Forward and Reverse Control Primer Mix||-20||10 µM each|
|Proteinase K, Molecular Biology Grade||-20||800 mg/ml|
|Uridine Diphosphate Glucose||-20||25 X|
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