WarmStart® LAMP Kit (DNA & RNA)

  • Simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets
  • Compatible with multiple detection methods
  • WarmStart® technology inhibits activity at room temperature

Ordering Information

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  • 100 reactions
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  • 500 reactions
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  • Product Information
    The WarmStart LAMP Kit (DNA & RNA) is designed to provide a simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets. LAMP and RT-LAMP are commonly used isothermal amplification techniques that provides rapid detection of a target nucleic acid using LAMP-specific primers (supplied by the user) and a strand-displacing DNA polymerase. This kit is supplied with the WarmStart LAMP 2X Master Mix, which contains a blend of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized LAMP buffer solution. Both Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase have been engineered for improved performance in LAMP and RT-LAMP reactions. A fluorescent dye is also supplied to enable real-time fluorescence measurement of LAMP. The WarmStart LAMP Kit is compatible with multiple detection methods, including turbidity detection, real-time fluorescence detection (when used with LAMP fluorescent dye) and end-point visualization.

    NEB’s WarmStart LAMP Kit (DNA & RNA) is compatible with multiple detection methods


    The NEB WarmStart LAMP Kit (DNA & RNA) includes separate fluorescent dye for real-time fluorescence measurement. Alternately, detection can be accomplished by turbidity detection or endpoint visualization.


    NEB’s WarmStart LAMP Kit (DNA & RNA) offers speed and robust sensitivity


    speed and sensitivity

    A RNA target (HMBS2) was amplified from Jurkat total RNA using the WarmStart LAMP Kit and OptiGene Master Mix (ISO-001). Reactions were performed at 65°C for 74 minutes on a real-time thermocycler (Bio-Rad CFX96) in triplicate. Time to result is set as the time at which the fluorescence crossed a threshold of 10% of maximal fluorescence. NEB’s WarmStart LAMP Kit resulted in faster and more robust detection as compared to the OptiGene Master Mix.


    NEB’s WarmStart LAMP Kit (DNA & RNA) provides fast LAMP/RT-LAMP results


    Fast LAMP/RT-LAMP results

    Experiments were designed to amplify a DNA target (BRCAb) and a RNA target (HMBS2) by LAMP or RT-LAMP, respectively. A 25 μl reaction containing 10 ng HeLa DNA or 5 ng Jurkat total RNA, 1X Master Mix, 1X LAMP primers and fluorescent dye was incubated on a Bio-Rad CFX98 instrument at 65°C for 75 minutes. Lucigen® OmniAmp® RNA & DNA Kit and OptiGene Isothermal Master Mix (ISO-001 ) were run according to manufacturers’ recommendations.

    NEB WarmStart LAMP Kit (DNA & RNA) enables specific amplification across multiple species/targets

    PRIMER SET

    DNA TEMPLATE

    HeLa 
    DNA

    Lyme 
    DNA

    B. malayi 
    DNA

    E. coli
    DNA

    C. elegans 
    DNA

    BRCAb (human)

    +

     

     

     

     

    CFTR (human)

    +

     

     

     

     

    LymO (Lyme)

     

    +

     

     

     

    BmHhal (B. malayi)

     

     

    +

     

     

    dnaE (E. coli)

     

     

     

    +

     

    Lec10b (C. elegans)

     

     

     

     

    +

    Lec6 (C. elegans)

     

     

     

     

    +

    In each individual reaction, multiple gDNA templates were added along with primers specific to a target in only one species. Reactions were incubated at 65°C for 74 minutes on a thermocycler (Bio-Rad CFX96) in triplicate. Positive results (+) were observed only in the expected species/target combinations, demonstrating high specificity of the WarmStart LAMP reactions.

    Kit Components

    The following reagents are supplied with this product:

    Store at (°C) Concentration
    LAMP Fluorescent Dye (50X) -20
    WarmStart LAMP 2X Master Mix -20
    Product Categories:
    Isothermal Amplification & Strand Displacement
    Applications:
    Isothermal Amplification
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