Easy cloning of all PCR products, including blunt and TA ends
This PCR Cloning Kit contains an optimized Cloning Mix containing a proprietary ligation enhancer and a linearized vector that uses a novel mechanism for background colony suppression to give a low background. It allows simple and quick cloning of any PCR amplicon, whether the amplification reactions are performed with proofreading DNA polymerases, such as Q5® which produce blunt ends; or nonproofreading DNA polymerases, such as Taq or Taq mixes (OneTaq®, LongAmp® Taq) which produce single base overhangs. This is possible due to “invisible” end polishing components in the master mix that are active during the ligation step only if needed. The kit also allows direct cloning from amplification reactions without purification, and works well whether or not the primers used in the PCR possess 5´-phosphate groups. To learn more about how the kit works, please view our video.
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|NEB® 10-beta/Stable Outgrowth Medium||4||1 X|
|NEB® 10-beta Competent E. coli (Cloning Efficiency)||-80|
|Cloning Mix 1||-20||2.5 X|
|Cloning Mix 2||-20||10 X|
|Linearized pMiniT™ 2.0 Vector||-20||25 µg/ml|
|Amplicon Cloning Control||-20||15 ng/µl|
|pUC19 Vector||-20||0.05 ng/µl|
|Cloning Analysis Forward Primer||-20||100 µM|
|Cloning Analysis Reverse Primer||-20||100 µM|
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