The K. lactis Protein Expression Kit provides an easy method for expressing a gene of interest in the yeast Kluyveromyces lactis. Proteins may be produced intracellularly or secreted using an integrative expression vector (pKLAC2). To achieve protein secretion, a gene of interest is cloned downstream of the K. lactis α-mating factor secretion domain, which is eventually processed in the Golgi and results in secretion of the desired protein.
The following reagents are supplied with this product:
Store at (°C) | Concentration | |
CutSmart® Buffer | -20 | 10 X |
Yeast Carbon Base Medium Powder | 4 | |
Acetamide solution (sterile) (10 ml) | 4 | 100 X |
K. lactis GG799 Competent Cells | -80 | 5 reactions |
NEB® Yeast Transformation Reagent (5 ml) | 4 | 1 X |
pKLAC1-malE Control Plasmid | -20 | 1,000 µg/ml |
pKLAC2 Vector | -20 | 1,000 µg/ml |
SacII | -20 | 20,000 units/ml |
Integration Primer 1 | -20 | 10 X |
Integration Primer 2 | -20 | 10 X |
Integration Primer 3 | -20 | 10 X |
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