K. lactis Protein Expression Kit

We are excited to announce that we are in the process of switching all reaction buffers to be BSA-free. Beginning April 2021, we will be gradually transitioning to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. All information on the website has been updated to reflect this change. Find more details at www.neb.com/BSA-free.

The K. lactis Protein Expression Kit provides an easy method for expressing a gene of interest in the yeast Kluyveromyces lactis. Proteins may be produced intracellularly or secreted using an integrative expression vector (pKLAC2). To achieve protein secretion, a gene of interest is cloned downstream of the K. lactis α-mating factor secretion domain, which is eventually processed in the Golgi and results in secretion of the desired protein.

  • Supplied with competent K. lactis cells (NEB# C1001)
  • Allows cloning and expression of genes toxic to E. coli
  • Rapid high cell density growth results in high yield protein expression 
  • Easy-to-use protocols for those inexperienced with yeast systems
  • No expensive antibiotics or methanol required
  • Attractive commercial sub-licensing
Catalog # Concentration Size
E1000S Not Applicable 1 set
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