New England Biolabs is working diligently to ensure we keep our employees and their families safe, while maintaining our business continuity. Despite a "Stay At Home" advisory being put in place in Massachusetts, USA, we are deemed an essential business, and our manufacturing and distribution teams continue to be fully operational. We are continuing to work with our suppliers and distribution partners to ensure uninterrupted access to our products and technical support.

Q5® Site-Directed Mutagenesis Kit

The Q5® Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours.

  • Non-overlapping primer design ensures robust, exponential amplification, generating a high percentage of desired mutations from a wide range of templates
  • Intramolecular ligation and transformation into NEB high-efficiency competent cells results in high colony yield
  • Extremely low error rate of Q5 Hot Start High-Fidelity DNA Polymerase reduces screening time
  • Hot start polymerase enables room temperature reaction set up
  • DpnI background reduction permits a wide range of starting template concentrations
  • Use of standard primers eliminates additional expenses from phosphorylated or purified oligos
  • Easy-to-use PCR master mix and unique multi-enzyme KLD Mix offer convenience and quality
  • Rapid and direct treatment step proceeds at room temperature in 5 minutes
  • Allows the use of any chemically-competent E. coli cells suitable for cloning
  • Supplied with competent cells 
  • Use NEBaseChanger™ tool to generate primer sequences and an annealing temperature
Learn more about how this product is being used in the Product Citation Tool
Catalog # Concentration Size
E0554S Not Applicable 10 reactions
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