Q5® Site-Directed Mutagenesis Kit

The Q5® Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours.

  • Non-overlapping primer design ensures robust, exponential amplification, generating a high percentage of desired mutations from a wide range of templates
  • Intramolecular ligation and transformation into NEB high-efficiency competent cells results in high colony yield
  • Extremely low error rate of Q5 Hot Start High-Fidelity DNA Polymerase reduces screening time
  • Hot start polymerase enables room temperature reaction set up
  • DpnI background reduction permits a wide range of starting template concentrations
  • Use of standard primers eliminates additional expenses from phosphorylated or purified oligos
  • Easy-to-use PCR master mix and unique multi-enzyme KLD Mix offer convenience and quality
  • Rapid and direct treatment step proceeds at room temperature in 5 minutes
  • Allows the use of any chemically-competent E. coli cells suitable for cloning
  • Supplied with competent cells 
  • Use NEBaseChanger™ tool to generate primer sequences and an annealing temperature
Learn more about how this product is being used in the Product Citation Tool
Catalog # Concentration Size
E0554S Not Applicable 10 reactions
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