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NEB offers a number of endonucleases and exonucleases that cleave DNA.

Exonucleases catalyze the removal of nucleotides in either the 5’ to 3’ or the 3’ to 5’ direction from single or double stranded DNA. Endonucleases cleave DNA nonspecifically and can be used to generate blunt ends for ligation or to shorten duplex DNA.

Learn more about common applications and properties of exonucleases and endonucleases.


  1. Kunitz, M. (1950) J. Gen. Physiol. 33, 349-362. PMID: 15406373
  2. Vanecko, S. and laskowski, M. (1961) J. Biol. Chem. 236, 3312-3316. PMID: 13924728
  3. Cuatrecasas, S.F., and Anfinsen, C.B. (1967) J. Biol. Chem., 242, 1541-1547. PMID: 4290246

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Protocols for Nucleases
Application Notes for Nucleases
Common Applications of Exonucleases and Endonucleases
NEB provides a list of common applications for our exonucleases and endonucleases.
Properties of Exonucleases and Endonucleases
NEB supplies many nucleases; several characteristics should be considered when choosing the one best suited to your particular research needs.