DNA Repair Enzymes and Structure-specific Endonucleases are enzymes which cleave DNA at a specific DNA lesion or structure. (To learn about non-specific endonucleases and exonucleases, visit here.) These enzymes can be used in a wide variety of applications such as:
- Repair of DNA sample degradation due to oxidative damage, UV radiation, ionizing radiation, phenol/chloroform extraction, mechanical shearing, formalin fixation (post extraction) or long term storage
- Base excision repair (BER)
- DNA mismatch repair
- Nucleotide excision repair
- Forensic analysis of environmental samples, analysis of ancient DNA, DNA damage control, and DNA-DNA and protein-DNA interactions
- Preparation for downstream applications such as PCR, microarray analysis, or other DNA technologies
- Single cell gel electrophoresis (Comet assay) to assess samples for DNA damage
- Genotoxicity tests by alkaline elution or alkaline unwinding
- Elimination or repair of large DNA secondary structures using T7 Endonuclease I (NEB #M0302)
- Comet Assay - Modified for Detection of Oxidized Bases Using the Repair Endonucleases Fpg, hOGG1 and Endonuclease III (Nth)
- Control Reaction Protocol for PreCR Repair Mix
- Sequential Reaction Protocol for PreCR Repair Mix
- Standard E. coli DNA Gyrase Reaction (M0306)
- Standard Reaction Protocol for PreCR Repair Mix
- Using RecA and an oligonucleotide to form a stable triple helix
- Determining Genome Targeting Efficiency using T7 Endonuclease I
- T7 Endonuclease I-based Mutation Detection with the EnGen® Mutation Detection Kit (NEB #E3321)
- Transfection of Cas9 RNP (ribonucleoprotein) into adherent cells using the Lipofectamine® RNAiMAX
DNA Damage - the major cause of missing pieces from the DNA puzzle
- Activities of DNA Repair Enzymes and Structure-specific Endonucleases
- Properties of DNA Repair Enzymes and Structure-specific Endonucleases
- DNA Damage and PreCR
- Karbaschi, Mahsa., Macip, Salvador., Mistry, Vilas., Abbas, Hussein H.K., Delinassios, George J., Evans, Mark D., Young, Antony R., Cooke, Marcus S. 2015. Rescue of cells from apoptosis increases DNA repair in UVB exposed cells: implications for the DNA damage response Toxicol. Res.. 4, PubMedID: , DOI: doiL 10.1039/c4tx00197d
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Learn about endonucleases and some of their common applications.
This webinar reviews the activities of exonucleases and endonucleases, provides insight on how to choose the right exo- or endonuclease for various molecular biology applications, and explains how to use these reagents when developing new molecular biology workflows.