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DNA Modifying Enzymes & Cloning Technologies

Advances in molecular biology require constant innovation and forward progress in the tools and techniques at the core of the field. New England Biolabs is committed to providing a wide variety of essential reagents that serve to advance the frontier of DNA-based manipulations.

NEB’s enzymology expertise sets it apart from competitors, allowing us to produce enzymes for molecular biology that deliver; our highly pure enzymes, over 250 of which are recombinant, offer exceptional performance and value.

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DNA Modifying Enzymes & Cloning Technologies includes these subcategories:
DNA Ligase Products
DNA Repair Enzymes and Structure-specific Endonucleases
Exonucleases and Non-specific Endonucleases
Phosphatases & Sulfurylases
ssDNA Binding Proteins
DNA Labeling
Other Products
Protocols for DNA Modifying Enzymes & Cloning Technologies
Application Notes for DNA Modifying Enzymes & Cloning Technologies
    Publications related to DNA Modifying Enzymes & Cloning Technologies
  1. Pirone-Davies, C., Hoffmann, M., Roberts, R.J., Muruvanda, T., Timme, R.E., Strain, E., Luo, Y., Payne, J., Luong, K., Song, Y., Tsai, Y.C., Boitano, M., Clark, T.A., Korlach, J., Evans, P.S., Allard, M.W. 2015. Genome-Wide Methylation Patterns in Salmonella enterica Subsp. enterica Serovars PLoS One. 10(4), PubMedID: 25860355, DOI:
  2. Seib, K.L., Jen, F.E., Tan, A., Scott, A.L., Kumar, R., Power, P.M., Chen, L.T., Wu, H.J., Wang, A.H., Hill, D.M., Luyten, Y.A., Morgan, R.D., Roberts, R.J., Maiden, M.C., Boitano, M., Clark, T.A., Korlach, J., Rao, D.N. and Jennings, M.P. 2015. Specificity of the ModAll, ModA12 and ModD1 epigenetic regulator N6-adenine DNA methyltransferases of Neisseria Meningitidis Nucleic Acids Res. 43(8), PubMedID: 25845594, DOI: 10.1093/nar/gkv219
  3. Fomenkov, A., Lunnen, K.D., Zhu, Z., Anton, B.P., Wilson, G.G., Vincze, T. and Roberts, R.J. 2015. Complete genome sequence and methylome analysis of bacillus strain x1 Genome Announc. 3(1), PubMedID: 25700417, DOI:
  4. Anton, B.P., Mongodin, E.F., Agrawal, S., Fomenkov, A., Byrd, D.R., Roberts, R.J. and Raleigh, E.A. 2015. Complete Genome Sequence of ER2796, a DNA Methyltransferase-Deficient Strain of Escherichia coli K-12 PLoS One. 10(5), PubMedID: 26010885, DOI:
  5. Xu, S-Y., Boitano, M., Clark, T.A., Vincze, T., Fomenkov, A., Kumar, S., Too, PH-M., Gonchar, D., Degtyarev, S.K. and Roberts, R.J. 2015. Complete Genome Sequence Analysis of Bacillus Subtilis T30 Genome Announc. 3(3), PubMedID: 25953183, DOI:
  6. Pais, J.E., Dai, N., Tamanaha, E., Vaisvila, R., Fomenkov, A.I., Bitinaite, J., Sun, Z., Guan, S., Correa, I.R. Jr., Noren, C.J., Cheng, X., Roberts, R.J., Zheng, Y. and Saleh, L. 2015. Biochemical characterization of a Naegleria TET-like oxygenase and its application in single molecule sequencing of 5-methylcytosine Proc Natl Acad Sci U S A. 112(14), PubMedID: 25831492, DOI:
  7. Mauris, J.and Evans, T.C., Jr. 2010. A human PMS2 homologue from Aquifex aeolicus stimulates an ATP-dependent DNA helicase. J Biol Chem. 285(15), PubMedID: 20129926, DOI:
  8. Roberts, R.J., Vincze, T., Posfai, J., Macelis, D. 2014. REBASE - A database for DNA restriction and modification: enzymes, genes and genomes Nucleic Acids Res. , PubMedID: , DOI:
  9. Deptula, P., Smolander, O.-P., Laine, P., Roberts, R.J., Edelmann, M., Peltola, P., Piironen, V., Paulin, L., Storgards, E., Savijoki, K., Laitila, A., Auvinen, P., Varmanen, P 2018. Acidipropionibacterium virtanenii sp nov isolated from malted barley Int J Syst Evol Microbiol. 68, PubMedID: 30156530, DOI:
  10. Anton, B.P., Roberts, R.J., Fomenkov, A., Humbert, A., Stoian, N., Zeilstra-Ryalls, J. 2018. Complete genome sequences of two Rhodobacter strains Microbiol Resour Announc. 7 (12), PubMedID: 30533667, DOI:
  11. Fomenkov, A., Vincze, T., Mersha, F., Roberts, R.J. 2018. Complete genome sequence and methylome analysis of Bacillus caldolyticus NEB414. Genome Announ.. 6 (6), PubMedID: 29439055 , DOI: 10.1128/genomeA.01605-17
  12. Bottacini, F., Morrissey, R., Roberts, R.J., James, J., van Breen, J., Egan, M., Lambert, J., van Limpt, K., Knol, J., O'Connell Motherway, M. and van Sinderen, D. 2018. Comparative genome and methylome analysis reveals restriction/modification system diversity in the gut commensal Bifidobacterium breve. Nucleic Acids Res. 46(4), PubMedID: 29294107, DOI: 10.1093/nar/gkx1289
  13. Murray, Iain; Morgan, Rick; Luyten, Yvette; Fomenkov, Alexey; Correa, Ivan; Dai, Nan; Roberts, Rich; 2018. The non-specific adenine DNA methyltransferase M.EcoGII. Nucleic Acids Res. (2), PubMedID: 29228259, DOI: 10.1093/nar/gkx1191
  14. Callahan, S.J., Luyten, Y.A., Gupta, Y.K., Wilson, G.G., Roberts, R.J., Morgan, R.D. and Aggarwal, A.K. 2016. Structure of Type IIL Restriction-Modification Enzyme MmeI in Complex with DNA Has Implications for Engineering New Specificities. PLoS Biol. Apr 15;14(4):e1002442, PubMedID: 27082731, DOI:
  15. Morgan, R.D., Luyten, Y.A., Johnson, S.A., Clough, E.M., Clark, T.A. and Roberts, R.J. 2016. Novel m4C modification in type I restriction-modification systems. Nucleic Acids Res. Nov, PubMedID: 27580720, DOI:
  16. Blow, M.J., Clark, T.A., Daum, C.G., Deutschbauer, A.M., Fomenkov, A., Fries, R., Froula, J., Kang, D.D., Malmstrom, R.R., Morgan, R.D., Posfai, J., Singh, K., Visel, A., Wetmore, K., Zhao, Z., Rubin, E.M., Korlach, J., Pennacchio, L.A. and Roberts, R.J. 2016. The Epigenomic Landscape of Prokaryotes. PLoS Genet. Feb 12;12(2):e1005854, PubMedID: 26870957, DOI: 10.1371/journal.pgen.1005854
  17. Ee, R., Lim, Y.L., Yin, W.F., See-Too, W.S., Roberts, R.J. and Chan, K.G. 2016. Novel Methyltransferase Recognition Motif Identified in Chania multitudinisentens RB-25(T) gen. nov., sp. nov. Front Microbiol. 206, PubMedID: 27630623 , DOI: 10.3389/fmicb.2016.01362
  18. Yao, K., Muruvanda, T., Roberts, R.J., Payne, J., Allard, M.W. and Hoffmann, M. 2016. Complete Genome and Methylome Sequences of Salmonella enterica subsp. enterica Serovar Panama (ATCC 7378) and Salmonella enterica subsp. enterica Serovar Sloterdijk (ATCC 15791). Genome Announc. Mar 17;4(2)., PubMedID: 26988049, DOI:
  19. Anton, B.P., Harhay, G.P., Smith, T.P., Blom, J. and Roberts, R.J. 2016. Comparative Methylome Analysis of the Occasional Ruminant Respiratory Pathogen Bibersteinia trehalosi. PLoS One. Aug 24, PubMedID: 27556252 , DOI:
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