NEB’s enzymology expertise sets it apart from competitors, allowing us to produce enzymes for molecular biology that deliver; our highly pure enzymes, over 250 of which are recombinant, offer exceptional performance and value.
- Sample preparation using provided buffer (N0364)
- Optimizing Restriction Endonuclease Reactions
- Double Digest Protocol with Standard Restriction Enzymes
- NEBuilder HiFi DNA Assembly Reaction Protocol
- NEBuilder® HiFi DNA Assembly Electrocompetent Transformation Protocol
- NEBuilder® HiFi DNA Assembly Chemical Transformation Protocol
- HiFi Taq DNA Ligase (M0647) Protocol
- Protocol for a substrate with 5-hydroxymethyluridine such as bacteriophage SP8 genomic DNA (M0659)
- Choosing the Right Exonuclease
- Quick Tips - Can I make multiple mutations with the Q5 site-directed mutagenesis kit?
- DNA Ligase Selection Chart
- Properties of DNA and RNA Ligases
- Troubleshooting Guide for Cloning
- Troubleshooting Tips for Ligation Reactions
- Tips for Maximizing Ligation Efficiencies
- Traditional Cloning Quick Guide
- A novel enzyme isolated from a hot spring metagenomic DNA library defines a new CAZy family (GH154) of exosialidases with an inverting catalytic mechanism (2018)
- Choosing the Right Exonuclease (2019)
- Single-Strand Specific, Plasmid Borne DNA Methyltransferases M.BceJIII and M.EcoGIX Regulate Plasmid and Single-Stranded Phage Replication. (2017)
- miRNA Detection by Ligation and Amplification of Complementary DNA Oligos Using SPlintR® Ligase (2015)
- Martinez, F.L., Anton, B.P., DasSharma, P., Pajal, V., Irazusta, V., Roberts, R.J., DasSharma, S. 2019. Complete genome sequence and methylome analysis of micrococcus luteus SA211, a halophilic, lithium-tolerant Actinobacterium from Argentina Microbiol Resour Announc. 8 (4), PubMedID: 30701250, DOI: 10.1128/MRA.01557-18
- Mauris, J.and Evans, T.C., Jr. 2010. A human PMS2 homologue from Aquifex aeolicus stimulates an ATP-dependent DNA helicase. J Biol.Chem. 285(15), PubMedID: 20129926, DOI:
- Roberts, R.J., Vincze, T., Posfai, J., Macelis, D. 2014. REBASE - A database for DNA restriction and modification: enzymes, genes and genomes Nucleic Acids Research, Advance Access . , PubMedID: , DOI:
- Pirone-Davies, C., Hoffmann, M., Roberts, R.J., Muruvanda, T., Timme, R.E., Strain, E., Luo, Y., Payne, J., Luong, K., Song, Y., Tsai, Y.C., Boitano, M., Clark, T.A., Korlach, J., Evans, P.S., Allard, M.W. 2015. Genome-Wide Methylation Patterns in Salmonella enterica Subsp. enterica Serovars PLoS One. 10(4), PubMedID: 25860355, DOI:
- Seib, K.L., Jen, F.E., Tan, A., Scott, A.L., Kumar, R., Power, P.M., Chen, L.T., Wu, H.J., Wang, A.H., Hill, D.M., Luyten, Y.A., Morgan, R.D., Roberts, R.J., Maiden, M.C., Boitano, M., Clark, T.A., Korlach, J., Rao, D.N. and Jennings, M.P. 2015. Specificity of the ModAll, ModA12 and ModD1 epigenetic regulator N6-adenine DNA methyltransferases of Neisseria Meningitidis Nucleic Acids Res. 43(8), PubMedID: 25845594, DOI:
- Morgan, R.D., Luyten, Y.A., Johnson, S.A., Clough, E.M., Clark, T.A. and Roberts, R.J. 2016. Novel m4C modification in type I restriction-modification systems. Nucleic Acids Res. . Nov, PubMedID: 27580720, DOI:
- Ee, R., Lim, Y.L., Yin, W.F., See-Too, W.S., Roberts, R.J. and Chan, K.G. 2016. Novel Methyltransferase Recognition Motif Identified in Chania multitudinisentens RB-25(T) gen. nov., sp. nov. Front Microbiol . 206, PubMedID: 27630623 , DOI:
- Fomenkov, A., Vincze, T., Mersha, F., Roberts, R.J. 2018. Complete genome sequence and methylome analysis of Bacillus caldolyticus NEB414. Genome Announcements. 6 (6), PubMedID: 29439055 , DOI: 10.1128/genomeA.01605-17
- Fomenkov, A., Lunnen, K.D., Zhu, Z., Anton, B.P., Wilson, G.G., Vincze, T. and Roberts, R.J. 2015. Complete genome sequence and methylome analysis of bacillus strain x1 Genome Announc. 3(1), PubMedID: 25700417, DOI:
- Anton, B.P., Mongodin, E.F., Agrawal, S., Fomenkov, A., Byrd, D.R., Roberts, R.J. and Raleigh, E.A. 2015. Complete Genome Sequence of ER2796, a DNA Methyltransferase-Deficient Strain of Escherichia coli K-12 PLoS One. 10(5), PubMedID: 26010885, DOI:
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