The largest selection of DNA Ligases for your ligation needs
With over 40 years of experience in the development and production of enzymes for molecular biology, NEB offers the most extensive selection of high-quality and performance-optimized DNA ligases and master mixes to streamline your experiments.
A few of our more popular standalone ligases and ligase kits available are:
Electroligase® is a registered trademark of New England Biolabs.
Quick Ligation™ is a trademark of New England Biolabs
SilverXpress™is a trademark of Life Technologies, Inc.
- Ligation Protocol with T4 DNA Ligase (M0202)
- NEBNext Quick Ligation Module Protocol (E6056)
- Quick Ligation Protocol (M2200)
- Transformation Protocol
- Transformation Protocol (M0367)
- Transformation Protocol (M0370)
- Ligation Protocol for Cloning with Instant Sticky-end Ligase Master Mix (M0370)
- Ligation Protocol for Cloning with Blunt/TA Ligase Master Mix (M0367)
- Ligation Protocol for Cloning with ElectroLigase® (M0369)
- Transformation Protocol (M0369)
- Protocol for ssDNA/RNA Ligation (M0319)
- Ligation Protocol with T3 DNA Ligase (M0317)
- Ligation Protocol with T7 DNA Ligase (M0318)
- Ligation protocol using SplintR® Ligase (M0375)
- E. coli DNA Ligase Protocol (M0205)
- Protocol for 9°N DNA Ligase (M0238)
- Protocol for Taq DNA Ligase (M0208)
- HiFi Taq DNA Ligase (M0647) Protocol
- Protocol for Salt-T4® DNA Ligase (NEB #M0467)
- Protocol for Hi-T4™ DNA Ligase (NEB #M2622)
- DNA Ligase Brochure
- DNA Ligase Selection Chart
- Properties of DNA and RNA Ligases
- Troubleshooting Guide for Cloning
- Troubleshooting Guide for Ligases
- Troubleshooting Tips for Ligation Reactions
- Tips for Maximizing Ligation Efficiencies
- Traditional Cloning Quick Guide
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
High fidelity polymerases are everywhere—but why would you need a high fidelity ligase? And what do we even mean by “fidelity” when we’re talking about ligation? In this webinar, NEB Scientist and ligase expert Greg Lohman discusses mismatch ligation by DNA ligases and the molecular diagnostics applications that depend on the use of high-fidelity DNA ligases like NEB’s HiFi Taq DNA Ligase to detect single base differences in DNA.
Ligation, the process of joining DNA fragments with a DNA ligase, proceeds in three steps. Learn more about the function of ligation with our quick tutorial animation.
Ligation of blunt ends and single-base overhangs require optimized reaction conditions.
Polyethylene glycol (PEG) is an important reagent in ligation reactions, find out why.
Find out how the downstream application dictates the best reaction conditions for ligation.
The optimal reactant ratio is contingent upon the downstream application.